Product Name: Nek7-pT191+S195
Product Number: AB-PK735
Size: 25 µg      Price:89.00
      $US
Target Full Name: NIMA (never-in-mitosis)-related protein-serine kinase 7

Target Alias: Kinase Nek7; NIMA (never in mitosis gene a)-related kinase 7; NIMA-related serine/threonine kinase Nek7

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK735

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T191+S195

Protein UniProt: Q8TDX7

Protein SigNET: Q8TDX7

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human Nek7 sequence peptide Cat. No.: PE-04AHR99
Antibody Immunogen Sequence: (pT)AAH(pS)LVG(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues T191 to G198; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.5 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 34-37 kDa.

Related Product 1: Nek7-pT191+S195 blocking peptide

Scientific Background: Nek7 is a protein-serine/threonine kinase that is a member of the Other group of protein kinases in the Nek family. This kinase is highly expressed and widely distributed in most tested human tissues. Nek7 is a member of the NIMA family of serine/threonine kinases. In contrast to the other documented NimA-related kinases, Nek7 harbor its catalytic domain in the C-terminus of the protein. Immunofluorescence studies indicate that Nek7 is cytoplasmic and located on chromosome 1. The major protein kinase that is active on the p70 S6 kinase hydrophobic regulatory site (FXXFS/TF/Y) T412, was purified from rat liver and identified as Nek7. Nek7 phosphotransferase activity is rapidly and efficiently increased by serum deprivation, and may be regulated in a cell cycle-dependent manner. Nek7 has been linked with the development of ovarian serous carcinomas. Nek7 has been significantly associated with certain clinicopathologic indices in gallbladder carcinomas. The high level expression of Nek7, FoxM1 and Plk1 was significantly associated with shorter overall survival time in 76 specimens of gallbladder carcinomas, pericarcinomas and normal tissues (PubMEd: 23359173).
Figure 1. Epitope mapping of Nek7-pT191+S195 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to Nek7-pT191+S195.