Product Name: Mos-pY263
Product Number: AB-PK722
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Moloney sarcoma oncogene-encoded protein-serine kinase
Target Alias: ADRB2; C-mos; MGC119963; MSV; MGC119962; CCDS6164.1; ENSG00000172680
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK722
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y263
Protein UniProt: P00540
Protein SigNET: P00540
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Mos sequence peptide Cat. No.: PE-04AOC99
Target Alias: ADRB2; C-mos; MGC119963; MSV; MGC119962; CCDS6164.1; ENSG00000172680
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK722
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y263
Protein UniProt: P00540
Protein SigNET: P00540
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Mos sequence peptide Cat. No.: PE-04AOC99
Antibody Immunogen Sequence: ADI(pY)SFA(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues A260 to A266; In the protein kinase catalytic domain near subdomain IX.
Antibody Immunogen Description: Corresponds to amino acid residues A260 to A266; In the protein kinase catalytic domain near subdomain IX.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 40-45 kDa.
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 40-45 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in phenylarsine oxide (PAO)+vanadate-treated HeLa (homogenized in SDS-PAGE sample buffer), a strong ~160K cross-reactive protein in EGF-treated A431 cells (homogenized in SDS-PAGE sample buffer); In insulin-treated MCF7 cells, strong background >75 kDa was observed (homogenized in SDS-PAGE sample buffer).
Related Product 1: Mos-pY263 blocking peptide
Related Product 2: Mos (Mos-1) pan-specific antibody (Cat. No.: AB-NK112)
Antibody Cross Reactivity: No significant cross-reactive proteins detected in phenylarsine oxide (PAO)+vanadate-treated HeLa (homogenized in SDS-PAGE sample buffer), a strong ~160K cross-reactive protein in EGF-treated A431 cells (homogenized in SDS-PAGE sample buffer); In insulin-treated MCF7 cells, strong background >75 kDa was observed (homogenized in SDS-PAGE sample buffer).
Related Product 1: Mos-pY263 blocking peptide
Related Product 2: Mos (Mos-1) pan-specific antibody (Cat. No.: AB-NK112)
Scientific Background: Mos is a protein-serine/threonine kinase of the Other group and Mos family. During oocyte maturation, it acts as a kinase that directly phosphorylates and activates MEK1 and MEK2 in the MAP kinase signalling module. It is also known as "cytostatic factor" in unfertilized oocytes. It is activated by phosphorylation at S3, and inhibited by phosphorylation at S102. Mos was originally identified as an oncoprotein from avial retroviral studies. While it phosphorylates and activates MEK1 and MEK2, there is little evidence that it functions as an oncoprotein in human cancers.
Figure 1. Epitope mapping of Mos-pY263 antibody with similar phosphopeptides on dot blots.
Figure 2. Identification of phosphosites related to Mos-pY263.
© Kinexus Bioinformatics Corporation 2017