Product Name: MKK3-pS218
Product Number: AB-PK713
Size: 25 µg      Price:89.00
Target Full Name: MAPK/ERK protein-serine kinase 3 beta isoform (MKK3 beta); Dual specificity mitogen-activated protein kinase kinase 3

Target Alias: MAP kinase kinase 3; MAPK,ERK kinase 3; MAPK/ERK kinase 3; MAPKK 3; MAP2K3; MP2K3; PRKMK3; P46734; ENSG00000034152

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK713

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: S218

Protein UniProt: P46734

Protein SigNET: P46734

Antibody Type: Polyclonal

Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MKK3 (MAP2K3, MEK3) sequence peptide Cat. No.: PE-04ABS99

Antibody Immunogen Sequence: YLVD(pS)VAK(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues Y214 to K221; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.75 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 37-40 kDa.
Antibody Specificity: Very high

Antibody Cross Reactivity: Strongly immunoreactivity on protein dot blots with human recombinant MKK4, but not MEK1 and MKK6.

Related Product 1: MKK3-pS218 blocking peptide

Related Product 2: MKK3-1 pan-specific antibody (Cat. No.: AB-NK101-4)

Related Product 3: MKK3-2 pan-specific antibody (Cat. No.: AB-NK101-5)

Related Product 4: MKK3-3 pan-specific antibody (Cat. No.: AB-NK101-6)

Related Product 5: MKK3-pY230 phosphosite-specific antibody (Cat. No.: AB-PK714)

Scientific Background: MKK3 (MAP2K3, MEK3) is a Dual specificity protein kinase of the STE group and STE7 family. MKK3 is activated by cytokines and environmental stress in vivo. Phosphorylation of S218 and T222 increases its phosphotransferase activity. S218A and T222A substitutions are involved in inactivation. S218E and T222E mutations are associated in constitutive activation. It catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the activation T loops of the p38 MAP kinase isoforms. Defects in the MKK3 gene may be involved colon cancer.
Figure 1. Epitope mapping of MKK3-pS218 antibody with similar phosphopeptides on dot blots.

Figure 2. Identification of phosphosites related to MKK3-pS218.