Target Full Name: MAPK/ERK kinase kinase 2; Mitogen-activated protein kinase kinase kinase 2

Target Alias: Kinase MEKK2; M3K2; MAP/ERK kinase kinase 2; MAP3K2; MAPK/ERK kinase kinase 2; MAPKKK2; MEK kinase 2; MEKK 2; MEKK2B; ENSG00000169967

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK700

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: S239

Protein UniProt: Q9Y2U5

Protein SigNET: Q9Y2U5

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human MEKK2 (MAP3K2) sequence peptide Cat. No.: PE-04ANX90

Antibody Immunogen Sequence: RAQ(pS)YPD(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues R236 to D242; In the region between the PB1 domain and the protein kinase catalytic domain

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.