Target Full Name: MAP/microtubule affinity-regulating protein-serine kinase 3

Target Alias: Cdc25C- associated protein kinase 1; Cdc25C-associated protein kinase 1; CTAK1; C-TAK1; ELKL motif kinase 2; KP78; PAR1A; Protein kinase STK10; Ser/Thr protein kinase PAR-1; Serine/threonine protein kinase p78; Serine/threonine-protein kinase p78

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK697

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T507

Protein UniProt: P27448

Protein SigNET: P27448

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human MARK3 sequence peptide Cat. No.: PE-04ANR99

Antibody Immunogen Sequence: RRN(pT)YVC(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues R504 to C510; In the C-terminal quarter of the protein kinase. This is the major in vivo phosphorylation site in MARK3.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.