Target Full Name: MAP/microtubule affinity-regulating protein-serine kinase 1

Target Alias: KIAA1477; MAP/microtubule affinity-regulating kinase 1; MARK

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK694

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T215

Protein UniProt: Q9P0L2

Protein SigNET: Q9P0L2

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human MARK1 sequence peptide Cat. No.: PE-04AAT95

Antibody Immunogen Sequence: NKLD(pT)F(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues K212 to G218; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII. This is the major in vivo phosphorylation site in MARK1.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.