Product Name: MAPKAPK5-pT186
Product Number: AB-PK693
Size: 25 µg      Price:89.00
Target Full Name: MAP kinase-activated protein kinase 5

Target Alias: MAP kinase-activated protein kinase 5; MAPKAP kinase 5; PRAK

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK693

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T186

Protein UniProt: Q8IW41

Protein SigNET: Q8IW41

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human MAPKAPK5 (PRAK) sequence peptide Cat. No.: PE-04AAS95
Antibody Immunogen Sequence: PQF(pT)PYYV(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues P183 to V190; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.5 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 53-58 kDa.

Scientific Background: MAPKAPK5 is a protein-serine/threonine kinase of the CAMK group and MAPKAPK family. p38 alpha and beta-dependent phosphorylation increases its activity. It is activated by stress-related extracellular stimuli; such as H2O2, arsenite, anisomycin TNF alpha and also PMA and the calcium ionophore A23187; but to a lesser extent. In vitro, activated by SQSTM1. T182A substitution leads to no p38-beta/MAPK11-induced activation. In addition, T182D, S212D and L337G mutations are associated with higher protein kinase activity. It appears to be a tumour suppressor protein (TSP) that mediates Ras-induced senescence and phosphorylates p53/TP53. It also phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, and RHEB.
Figure 1. Epitope mapping of MAPKAPK5-pT186 antibody with similar phosphopeptides on dot blots.

Figure 2. Identification of phosphosites related to MAPKAPK5-pT186.