Target Full Name: Lymphocyte-oriented protein-serine kinase; Serine-threonine-protein kinase 10

Target Alias: GEK2; STK10; Lymphocyte-oriented kinase; PRO2729

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK686

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T952

Protein UniProt: O94804

Protein SigNET: O94804

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human LOK sequence peptide Cat. No.: PE-04ANM95

Antibody Immunogen Sequence: NPS(pT)PSK(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues N949 to K955; In the C-terminus of the kinase. This is the major in vivo site of phosphorylation in LOK.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.