Product Name: Ksr1-pS406
Product Number: AB-PK675
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Protein-serine kinase suppressor of Ras 1
Target Alias: CAP kinase; Ceramide-activated protein kinase; HB; HB protein; Ksr; Ksr1; Kinase suppressor of ras 1; Kinase suppressor of RAS-1; RSU2; ENSG00000141068
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK675
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S406
Protein UniProt: Q8IVT5
Protein SigNET: Q8IVT5
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Target Alias: CAP kinase; Ceramide-activated protein kinase; HB; HB protein; Ksr; Ksr1; Kinase suppressor of ras 1; Kinase suppressor of RAS-1; RSU2; ENSG00000141068
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK675
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S406
Protein UniProt: Q8IVT5
Protein SigNET: Q8IVT5
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Ksr1 sequence peptide Cat. No.: PE-04ANE99
Antibody Immunogen Sequence: RTE(pS)VPS(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R401 to S407; In the region between the Ksr1-SAM and kinase catalytic domains. This is the major in vivo phosphorylation site in Krs1.
Antibody Immunogen Sequence: RTE(pS)VPS(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R401 to S407; In the region between the Ksr1-SAM and kinase catalytic domains. This is the major in vivo phosphorylation site in Krs1.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 105-120 kDa.
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 105-120 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactivities in HepG2, Jurkat and T98G cells and in sea star oocytes, except for a ~65 kDa protein in HepG2 and T98G cells, and a strong 50 kDa protein in sea star oocytes.
Related Product 1: Ksr1-pS406 blocking peptide
Related Product 2: Ksr1 pan-specific antibody (Cat. No.: AB-NK090)
Related Product 3: Ksr2-pS490 phosphosite-specific antibody (Cat. No.: AB-PK676)
Antibody Cross Reactivity: No significant cross-reactivities in HepG2, Jurkat and T98G cells and in sea star oocytes, except for a ~65 kDa protein in HepG2 and T98G cells, and a strong 50 kDa protein in sea star oocytes.
Related Product 1: Ksr1-pS406 blocking peptide
Related Product 2: Ksr1 pan-specific antibody (Cat. No.: AB-NK090)
Related Product 3: Ksr2-pS490 phosphosite-specific antibody (Cat. No.: AB-PK676)
Scientific Background: Ksr1 (kinase suppressor of Ras 1) is a protein-serine/threonine kinase of the TKL group and RAF family. It is a Raf-related pseudokinase and may directly mediate Raf activation. It acts as a scaffold for the Raf/MEK/ERK kinase cascade. No specific mutations have been directly implicated in oncogenesis although loss of Ksr1 function will blunt the Ras pathway and therefore attenuate its oncogenic potential.
Figure 1. Epitope mapping of Ksr1-pS406 antibody with similar phosphopeptides on dot blots.
Figure 2. Identification of phosphosites related to Ksr1-pS406.
© Kinexus Bioinformatics Corporation 2017