Product Name: Kit-pY936
Product Number: AB-PK673
Size: 25 µg      Price:89.00
      $US
Target Full Name: Mast/stem cell growth factor receptor protein-tyrosine Kit

Target Alias: CD117; C-kit; Kinase Kit; Mast/stem cell growth factor receptor precursor; PBT; SCFR; SL; V-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene; V-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homologue; ENSG00000157404

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK673

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y936

Protein UniProt: P10721

Protein SigNET: P10721

Antibody Type: Polyclonal

Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Kit sequence peptide Cat. No.: PE-04AGB95

Antibody Immunogen Sequence: NHI(pY)SNL(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues N933 to L939; In the C-terminal of the kinase after the catalytic domain

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Very weak immunoreactivity of a target-sized protein by Western blotting in HeLa cells.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 125-145 kDa.
Antibody Specificity: High

Antibody Cross Reactivity: No significant cross-reactive proteins detected in HeLa and HepG2 cell, except for medium immunoreactivity with ~56, ~50 and ~37 kDa proteins.

Related Product 1: Kit-pY936 blocking peptide

Related Product 2: Kit-1 pan-specific antibody (Cat. No.: AB-NK241-1)

Related Product 3: Kit-2 pan-specific antibody (Cat. No.: AB-NK241-2)

Related Product 4: Kit -S821+Y823 phosphosite-specific antibody (Cat. No.: AB-PK674)

Scientific Background: Kit (SCFR) is a protein-tyrosine kinase of the TK group and PDGFR family. It is a receptor kinase that binds SCF/KitLG. Mast,stem cell growth factor (steel factor) binding induces Kit dimerization and autophosphorylation. Protein interactions are induced with phosphorylation at Y568 (with APS, Cbl, SHP2, & Src), Y900 (with Crk & PIK3C2A) and Y936 (with APS, Cbl, Grb2 & Grb7). Phosphorylation of S741 and S746 inhibits the kinase activity of Kit. Kit can mediate cell proliferation, survival, hematopoiesis, gametogenesis, stem cell maintenance, mast cell development, function, and migration, as well as melanogenesis. Some substrates that Kit can phosphorylate include PIK3R1, PLCG1, SH2B2/APS and CBL.This kinase is highly expressed and widely distributed in most tested human tissues. It was first identified as the cellular homologue of the feline sarcoma viral oncogene v-kit. Mutations in Kit are associated with gastrointestinal stromal tumors (GIST), mast cell leukemia, acute myelogenous leukemia (AML), and piebaldism. Kit has also been linked with the development of colorectal adenocarcinomas and melanoma progression. Insertional mutagenesis studies in mice also support a role for this protein kinase in mouse cancer oncogenesis.
Figure 1. Dot blotting Kit-pY936 antibody with recombinant purified proteins.


Figure 2. Epitope mapping of Kit-pY936 antibody with similar phosphopeptides on dot blots.


Figure 3. Epitope mapping of Kit-pY936 antibody with similar phosphopeptides on dot blots.


Figure 4. Identification of phosphosites related to Kit-pY936.