Product Name: GTF2F1-pS385+pT389
Product Number: AB-PK651
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: General transcription factor IIF subunit 1
Target Alias: BTF4; General transcription factor IIF polypeptide 1 74 kDa subunit protein; General transcription factor IIF, polypeptide 1 (74kD subunit); General transcription factor IIF, polypeptide 1, 74kDa; RAP74; T2FA; TF2F1; TFIIF; Transcription initiation factor IIF alpha subunit; Transcription initiation factor IIF subunit alpha; Transcription nitiation factor RAP74
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK651
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S385+T389
Protein UniProt: P35269
Protein SigNET: P35269
Target Alias: BTF4; General transcription factor IIF polypeptide 1 74 kDa subunit protein; General transcription factor IIF, polypeptide 1 (74kD subunit); General transcription factor IIF, polypeptide 1, 74kDa; RAP74; T2FA; TF2F1; TFIIF; Transcription initiation factor IIF alpha subunit; Transcription initiation factor IIF subunit alpha; Transcription nitiation factor RAP74
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK651
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S385+T389
Protein UniProt: P35269
Protein SigNET: P35269
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human GTF2F1 sequence peptide Cat. No.: PE-04AMS95
Antibody Immunogen Sequence: RGN(pS)RPG(pT)PSA(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R382 to A392; in the TFIIF_alpha domain
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human GTF2F1 sequence peptide Cat. No.: PE-04AMS95
Antibody Immunogen Sequence: RGN(pS)RPG(pT)PSA(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R382 to A392; in the TFIIF_alpha domain
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in maturing, germinal vesicle positive, sea star oocytes. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in maturing, germinal vesicle positive, sea star oocytes. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 66-74 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431, Jurkat and MCF7 cells, except for a strong immunoreactive ~75 kDa protein in FBS-treated A431 cells and germinal vesicle positive-maturing sea star oocytes.
Related Product 1: GTF2F1-pS385+pT389 blocking peptide
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431, Jurkat and MCF7 cells, except for a strong immunoreactive ~75 kDa protein in FBS-treated A431 cells and germinal vesicle positive-maturing sea star oocytes.
Related Product 1: GTF2F1-pS385+pT389 blocking peptide
Scientific Background: GTF2F1 is a protein-serine/threonine kinase of the Atypical group and family. It is also a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It can be inhibited by mutation of S385 and T389.
Figure 1. Epitope mapping of GTF2F1-pS385+pT389 antibody with similar phosphopeptides on dot blots.
Figure 2. Identification of phosphosites related to GTF2F1-pS385+pT389.
© Kinexus Bioinformatics Corporation 2017