Product Name: GSK3a-pT19+pS21
Product Number: AB-PK648
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Glycogen synthase-serine kinase 3 alpha
Target Alias: DKFZp686D0638; Factor A; Glycogen synthase kinase 3 alpha; GSK-3 alpha; GSK3-alpha; Kinase GSK3-alpha
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK648
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T19+S21
Protein UniProt: P49840
Protein SigNET: P49840
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human GSK3a sequence peptide Cat. No.: PE-04AMR99
Target Alias: DKFZp686D0638; Factor A; Glycogen synthase kinase 3 alpha; GSK-3 alpha; GSK3-alpha; Kinase GSK3-alpha
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK648
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T19+S21
Protein UniProt: P49840
Protein SigNET: P49840
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human GSK3a sequence peptide Cat. No.: PE-04AMR99
Antibody Immunogen Sequence: RAR(pT)S(pS)FAE(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R16 to E24; Near the N-terminus of the protein.
Antibody Immunogen Description: Corresponds to amino acid residues R16 to E24; Near the N-terminus of the protein.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in A431 cells and sea star oocytes. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in A431 cells and sea star oocytes. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-54 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431 and HeLa cells, and in sea star oocytes, except a strong cross-reactive 75 kDa protein was observed in A431 cells.
Related Product 1: GSK3a-pT19+pS21 blocking peptide
Related Product 2: GSK3a-BKCD pan-specific antibody (Cat. No.: AB-NK069-3)
Related Product 3: GSK3a-pS278+pY279 phosphosite-specific antibody (Cat. No.: AB-PK647)
Related Product 4: GSK3a-pY279 phosphosite-specific antibody (Cat. No.: AB-PK649)
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431 and HeLa cells, and in sea star oocytes, except a strong cross-reactive 75 kDa protein was observed in A431 cells.
Related Product 1: GSK3a-pT19+pS21 blocking peptide
Related Product 2: GSK3a-BKCD pan-specific antibody (Cat. No.: AB-NK069-3)
Related Product 3: GSK3a-pS278+pY279 phosphosite-specific antibody (Cat. No.: AB-PK647)
Related Product 4: GSK3a-pY279 phosphosite-specific antibody (Cat. No.: AB-PK649)
Related Product 5: GSK3a-pY284+Y285 phosphosite-specific antibody (Cat. No.: AB-PK650)
Related Product 6: GSK3Selectide - GSK3 protein kinase substrate peptide
Related Product 6: GSK3Selectide - GSK3 protein kinase substrate peptide
Scientific Background: GSK3a is a protein-serine/threonine kinase of the CMGC group and GSK family. It is highly active following tyrosine phosphorylation within its activation T loop. Phosphorylation at Y279 increases phosphotransferase activity and promotes nuclear localization. Phosphorylation at S21 inhibits phosphotransferase activity. Through direct phosphorylation, it can inactivate glycogen synthase (for insulin regulation), CTNNB1/beta-catenin, APC, and AXIN1. GSK3 has been proposed as a tumour suppressor protein (TSP), since with loss of GSK3 there is an increase in WNT signalling, with a beta-catenin stabilization, leading to tumourigenesis. GSK3 has also been proposed to act as an oncoprotein (OP), as acute leukemia required GSK3 for cell proliferation. In most human cancers, it is up-regulated in expression about 1.6-times the rate observed for most protein kinases.
Figure 1. Epitope mapping of GSK3a-pT19+pS21 antibody with similar phosphopeptides on dot blots.
Figure 2. Identification of phosphosites related to GSK3a-pT19+pS21.
© Kinexus Bioinformatics Corporation 2017