Product Name: ERK3-pS189
Product Number: AB-PK623
Size: 25 µg      Price:89.00
      $US
Target Full Name: Extracellular regulated protein-serine kinase 3

Target Alias: MAPK6; Mitogen-activated protein kinase 6; MK06; P55-MAPK; P97-MAPK; PRKM4; PRKM6; DKFZp686F03189; HsT17250; CCDS10147.1; ENSG00000069956

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK623

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: S189

Protein UniProt: Q16659

Protein SigNET: Q16659

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human ERK3 (MAPK6) sequence peptide Cat. No.: PE-04AKF90

Antibody Immunogen Sequence: GHL(pS)EGL(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues G186 to L192; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Very weak immunoreactivity of a target-sized protein by Western blotting in MCF7 cells.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 95-100 kDa.
Antibody Specificity: High-very high

Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431 cells, except a 75 kDa cross-reactive protein can be observed in both celll lnes.

Related Product 1: ERK3-pS189 blocking peptide

Scientific Background: ERK3 (MAPK6) is a protein-serine/threonine kinase of the CMGC group and MAPK family. ERK3 is highly expressed in the skeletal muscle and the brain. It is located in nucleus and has been shown to be activating in fibroblasts with serum or phorbol esters treatments. Phosphorylation at S189 increases its phosphotransferase activity. Overexpression of ERK3 in fibroblasts inhibits S-phase entry. It may act as a negative regulator of cell proliferation. It is downregulated in different tumour types. However regulation and function of ERK3 remains to be fully elucidated. It phosphorylates MAP2.
Figure 1. Dot blotting ERK3-pS189 antibody with recombinant purified proteins.


Figure 2. Epitope mapping of ERK3-pS189 antibody with similar phosphopeptides on dot blots.


Figure 3. Identification of phosphosites related to ERK3-pS189.