Target Full Name: Extracellular regulated protein-serine kinase 2 (p42 MAP kinase)

Target Alias: ERK; ERK-2; ERT1; Extracellular signal regulated kinase 2; Kinase ERK2; MAP kinase 2; MAP kinase isoform p42; MAPK; MAPK 2; PRKM2; p41mapk; CCDS13795.1; ENSG00000100030

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK622

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T185+Y187

Protein UniProt: P28482

Protein SigNET: P28482

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human ERK2 (MAPK1) sequence peptide Cat. No.: PE-04AGD95

Antibody Immunogen Sequence: GFL(pT)E(pY)V(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues G182 to V188; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.