Product Name: EphB2-pY780
Product Number: AB-PK610
Size: 25 µg      Price:89.00
      $US
Target Full Name: Ephrin type-B receptor 2 protein-tyrosine kinase

Target Alias: CEK5; DRT; Eph receptor B2; Eph3; Eph-3; EphB2; Ephrin type-B receptor 2; EphT3; Hek5; Tyro5; MGC87492; ENSG00000133216

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK610

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y780

Protein UniProt: P29323

Protein SigNET: P29323

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human EphB2 sequence peptide Cat. No.: PE-04ALZ95

Antibody Immunogen Sequence: DPT(pY)TSA(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues D777 to A783; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII. This is the major in vivo phosphorylation site in EphA2.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.5 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 100-115 kDa.
Antibody Specificity: High

Antibody Cross Reactivity: No significant cross-reactive proteins detected in MCF7 and HepG2 cells, except for a medium ~75 kDa cross-reactive protein in both cell lines.

Related Product 1: EphB2-pY780 blocking peptide

Related Product 2: EphB1-pY594 phosphosite-specific antibody (Cat. No.: AB-PK609)

Related Product 3: EphB2-BKCD pan-specific antibody (Cat. No.: AB-NK267-1)

Related Product 4: EphB2-PCT pan-specific antibody (Cat. No.: AB-NK267-2)

Related Product 5: EphB3-pY600 phosphosite-specific antibody (Cat. No.: AB-PK611)

Related Product 6: EphSubtide - EphB1 protein kinase substrate peptide
Scientific Background: EphB3 is a member of the TK group of protein kinases in the Eph family. EphB3 is expressed during embryonic development in multiple regions of the central nervous system. In adult brain, EphB3 is expressed in the cerebellum, raphe pallidus, hippocampus, entorhinal cortex, and both motor and sensory cortices, and is moderate to highly expressed in most tested adult human tissues. Orthologues of EphB3 are highly conserved in vertebrates, including amphibians. EphB3 is activated by binding ephrin-B1, B2, or B3. Phosphorylation at Y614 induces interaction with Crk, Fyn, and RasGAP. EphB3 is involved in the maintenance of mature neuronal connections and/or re-arrangement of synaptic connections during late stages of development. EphB3 plays a role in the regulation of cell adhesion and migration, and the catalytic activity of EphB3 is required for inhibition of integrin-mediated cell adhesion. EphB3 appears to be an oncoprotein (OP). Over-expression of EphB3 has been observed in several cancer types, including colon cancer and lung cancer. Additionally, EphB3 over-expression was demonstrated in non-small cell lung cancer (NSCLC) and the expression level was highly correlated with pathological features, including tumour size, differentiation, and metastasis. over-expression of EphB3 in NSCLC cell lines enhanced cell growth and migration and promoted tumourigenesis. By contrast, EphB3 knockout in NSCLC lines reduced cell proliferation and migration and suppressed tumour growth. EphB3 has been linked with the development of lung neuroendocrine carcinomas and lung small cell carcinomas (LSCC).
Figure 1. Epitope mapping of EphB2-pY780 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to EphB2-pY780.