Product Name: EphA3-pY779
Product Number: AB-PK608
Size: 25 µg      Price:89.00
      $US
Target Full Name: Ephrin type-A receptor 3 protein-tyrosine kinase

Target Alias: EPA3; Eph receptor A3; Ephrin type-A receptor 3; ETK; ETK1; HEK; HEK4; Kinase EphA3; Tyrosine-protein kinase receptor REK4; TYRO4; CCDS2922.1; ENSG00000044524

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK608

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y779

Protein UniProt: P29320

Protein SigNET: P29320

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human EphA3 sequence peptide Cat. No.: PE-04AGN65

Antibody Immunogen Sequence: EAA(pY)TTR(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues E776 to R782; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.4 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 120-130 kDa.

Related Product 1: EphA3-pY779 blocking peptide

Related Product 2: EphA1-pY781 phosphosite-specific antibody (Cat. No.: AB-PK605)

Related Product 3: EphA2-pY588 phosphosite-specific antibody (Cat. No.: AB-PK606)

Scientific Background: EphA3 is a protein-tyrosine kinase of the TK group and Eph family. It is a receptor kinase that binds ephrin ligands to induce contact signalling. It is activated by binding ephrin-A1, A2, A3, A4 or A5. Phosphorylation of Y596, Y602 and Y779 increases phosphotransferase activity and interaction with Crk. Upregulation of EphA3 is common in lung cancer and melanomas. Its mutation pattern in human tumours is very characteristic of a tumour suppressor protein.This pathway can regulate cell adhesion, migration, and movement. EphA3 function is inhibited and EFNA5 binding interrupted with the V133E, and F152L mutations. EphA3 is linked to Pleomorphic Rhabdomyosarcoma, which is a rare disease which is related to colorectal cancer, and can also affect the uterus, heart, and skeletal muscle tissues.
Figure 1. Identification of phosphosites related to EphA3-pY779.