Target Full Name: Eukaryotic translation initiation factor 2-alpha kinase 3

Target Alias: DKFZp781H1925; E2AK3; EIF2AK3; Eukaryotic translation initiation factor 2-alpha kinase 3; HsPEK; HRI; Kinase PEK; Pancreatic eIF2-alpha kinase; PRKR-like endoplasmic reticulum kinase

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK604

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T982

Protein UniProt: Q9NZJ5

Protein SigNET: Q9NZJ5

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human EIF2AK3 (PERK) sequence peptide Cat. No.: PE-04AON99

Antibody Immunogen Sequence: ARH(pT)GQV(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues A889 to V895; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.