Product Name: DNAPK-pY883
Product Number: AB-PK596
Size: 25 µg      Price:89.00
      $US
Target Full Name: DNA-dependent protein kinase catalytic subunit

Target Alias: DNA-PK catalytic subunit; DNA-PKcs; DNPK1; P460; PRKD; PRKDC; XRCC7; HYRC; HYRC1; p350; ENSG00000121031

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK596

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y883

Protein UniProt: P78527

Protein SigNET: P78527

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human DNAPK (PRKDC) sequence peptide Cat. No.: PE-04AFZ95

Antibody Immunogen Sequence: MKS(pY)VAW(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues M880 to W886; In the N-terminal quarter of the protein.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 460-480 kDa.

Related Product 1: DNAPK-pY883 blocking peptide

Related Product 2: DNAPK-pT2609 phosphosite-specific antibody (Cat. No.: AB-PK595)

Related Product 3: DNAPK (PRKDC) pan-specific antibody (Cat. No.: AB-NK048-3)

Scientific Background: DNAPK (PRKDC) is a protein-serine/threonine kinase of the Atypical group and PIKK family. Phosphorylation at -612, T2609, T2638, T2647 and T3950 inhibits phosphotransferase activity. DNAPK has been noted to be involved in sensing DNA damage, including DNA non-homologous end joining (NHEJ) and therefore, by association, be involved with V(D)J recombination events and double-strand break (DSB) repair. A possible scaffolding role of DNAPK for DNA-repair enzymes has also been proposed. DNAPK appears to be a tumour suppressor protein (TSP). The active form of the protein kinase normally acts to inhibit tumour cell proliferation until damage to DNA can be repaired. Therefore, it can play a role to facilitate tumour cell survival under conditions of radiation and chemotherapy.
Figure 1. Epitope mapping of DNAPK-pY883 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to DNAPK-pY883.