Product Name: DNAPK-pT2609
Product Number: AB-PK595
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: DNA-dependent protein kinase catalytic subunit
Target Alias: DNA-PK catalytic subunit; DNA-PKcs; DNPK1; P460; PRKD; PRKDC; XRCC7; HYRC; HYRC1; p350; ENSG00000121031
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK595
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T2609
Protein UniProt: P78527
Protein SigNET: P78527
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Target Alias: DNA-PK catalytic subunit; DNA-PKcs; DNPK1; P460; PRKD; PRKDC; XRCC7; HYRC; HYRC1; p350; ENSG00000121031
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK595
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T2609
Protein UniProt: P78527
Protein SigNET: P78527
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human DNAPK (PRKDC) sequence peptide Cat. No.: PE-04ALT95
Antibody Immunogen Sequence: FVT(pT)QAS(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues F2606 to S2612; In the mid-region of the protein kinase between the NUC194 and FAT domains.
Antibody Immunogen Sequence: FVT(pT)QAS(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues F2606 to S2612; In the mid-region of the protein kinase between the NUC194 and FAT domains.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 460-480 kDa.
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 460-480 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HeLa cells.
Related Product 1: DNAPK-pT2609 blocking peptide
Related Product 2: DNAPK-pY883 phosphosite-specific antibody (Cat. No.: AB-PK596)
Related Product 3: DNAPK (PRKDC) pan-specific antibody (Cat. No.: AB-NK048-3)
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HeLa cells.
Related Product 1: DNAPK-pT2609 blocking peptide
Related Product 2: DNAPK-pY883 phosphosite-specific antibody (Cat. No.: AB-PK596)
Related Product 3: DNAPK (PRKDC) pan-specific antibody (Cat. No.: AB-NK048-3)
Scientific Background: DNAPK (PRKDC) is a protein-serine/threonine kinase of the Atypical group and PIKK family. Phosphorylation at -612, T2609, T2638, T2647 and T3950 inhibits phosphotransferase activity. DNAPK has been noted to be involved in sensing DNA damage, including DNA non-homologous end joining (NHEJ) and therefore, by association, be involved with V(D)J recombination events and double-strand break (DSB) repair. A possible scaffolding role of DNAPK for DNA-repair enzymes has also been proposed. DNAPK appears to be a tumour suppressor protein (TSP). The active form of the protein kinase normally acts to inhibit tumour cell proliferation until damage to DNA can be repaired. Therefore, it can play a role to facilitate tumour cell survival under conditions of radiation and chemotherapy.
Figure 1. Epitope mapping of DNAPK-pT2609 antibody with similar phosphopeptides on dot blots.
Figure 2. Identification of phosphosites related to DNAPK-pT2609.
© Kinexus Bioinformatics Corporation 2017