Product Name: DDR2-pY740
Product Number: AB-PK594
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Discoidin domain-containing receptor 2
Target Alias: Discoidin domain receptor 2; Discoidin domain receptor tyrosine kinase 2; Neurotrophic tyrosine kinase, receptor-related 3; NTRKR3; Receptor protein-tyrosine kinase TKT; TKT; TYRO10; Tyrosine-protein kinase TYRO 10; MIG20a; ENSG00000162733
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK594
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y740
Protein UniProt: Q16832
Protein SigNET: Q16832
Antibody Type: Polyclonal
Target Alias: Discoidin domain receptor 2; Discoidin domain receptor tyrosine kinase 2; Neurotrophic tyrosine kinase, receptor-related 3; NTRKR3; Receptor protein-tyrosine kinase TKT; TKT; TYRO10; Tyrosine-protein kinase TYRO 10; MIG20a; ENSG00000162733
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK594
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y740
Protein UniProt: Q16832
Protein SigNET: Q16832
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human DDR2 (Tyro10) sequence peptide Cat. No.: PE-04AHA99
Antibody Immunogen Sequence: SGD(pY)YRI(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues S737 to I743; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Immunogen Source: Human DDR2 (Tyro10) sequence peptide Cat. No.: PE-04AHA99
Antibody Immunogen Sequence: SGD(pY)YRI(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues S737 to I743; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 90-95 kDa.
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 90-95 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HeLa and HepG2 cells, except for a ~75 kDa protein in both cell lines.
Related Product 1: DDR2-pY740 blocking peptide
Related Product 2: DDR2-pY736 phosphosite-specific antibody (Cat. No.: AB-PK593)
Related Product 3: DDR2Subtide - DDR2 protein kinase substrate peptide
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HeLa and HepG2 cells, except for a ~75 kDa protein in both cell lines.
Related Product 1: DDR2-pY740 blocking peptide
Related Product 2: DDR2-pY736 phosphosite-specific antibody (Cat. No.: AB-PK593)
Related Product 3: DDR2Subtide - DDR2 protein kinase substrate peptide
Scientific Background: DDR2 is a protein-tyrosine kinase of the TK group and DDR family. It is a receptor kinase that binds fibrillar collagen and has roles in the regulation of cell differentiation, extracellular matrix remodeling, cell migration, and cell proliferation. DDR2 is necessary for normal bone development and promotes fibroblast migration and proliferation, and has a critical role in wound healing. This kinase is highly expressed and widely distributed in most tested human tissues except in the brain where it is poorly expressed. Orthologues of DDR2 are highly conserved in vertebrates and insects. Phosphorylation of DDR2 at Y471 induces interaction with Shc1, whereas phosphorylation at Y740 induces interaction with Nck2 and SHP2. DDR2 isfeatures a distinct extracellular region encompassing a factor VIII-like domain, which is thought to be involved in the regulation of cell growth, differentiation, and metabolism. DDR2 plays a role in the regulation of collagen turnover mediated by smooth muscle cells in obstructive diseases of blood vessels and the lung. DDR2 has also been linked with the development of lung large cell carcinomas and spondyloepimetaphyseal dysplasia short limb-hand type (semd-sl).
Figure 1. Epitope mapping of DDR2-pY740 antibody with similar phosphopeptides on dot blots.
Figure 2. Identification of phosphosites related to DDR2-pY740.
© Kinexus Bioinformatics Corporation 2017