Product Name: CDK6-pY24
Product Number: AB-PK572
Size: 25 µg      Price:89.00
      $US
Target Full Name: Cyclin-dependent protein-serine kinase 6

Target Alias: CRK2; Cyclin-dependent kinase 6; Kinase CDK6; PLSTIRE; Serine/threonine protein kinase PLSTIRE; MGC59692; CCDS5628.1; ENSG00000105810

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK572

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y24

Protein UniProt: Q00534

Protein SigNET: Q00534

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human CDK6 sequence peptide Cat. No.: PE-04AJQ99

Antibody Immunogen Sequence: EGA(pY)GKV(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues E21 to V27; In the protein kinase catalytic domain in subdomain I.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.75 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 34-38 kDa.
Antibody Specificity: Very high

Antibody Cross Reactivity: No significant cross-reactive proteins detected in HepG2, HeLa and MCF7 cells and in sea star oocytes, except for medium ~75 kDa cross-reactive protein in HeLa cells.

Related Product 1: CDK6-pY24 blocking peptide

Related Product 2: CDK6-pY13 phosphosite-specific antibody (Cat. No.: AB-PK571)

Related Product 3: CDK4/6Selectide - CDK4 protein kinase substrate peptide

Scientific Background: CDK6 is a protein-serine/threonine kinase of the CMGC group and CDK family. It is involved in cell cycle regulation and differentiation, which promotes G1/S transition. Phosphorylation at T177 increases phosphotransferase activity. Germline mutations in CDK6 are hypothesized to lead to a predisposition to melanoma. A197T mutation is found in family with autosomal recessive primary microcephaly-12 (MCPH12).
Figure 1. Dot blotting CDK6-pY24 antibody with recombinant purified proteins.


Figure 2. Identification of phosphosites related to CDK6-pY24.