Product Name: CDK2-pT160
Product Number: AB-PK568
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Cyclin-dependent protein-serine kinase 2
Target Alias: Cyclin-dependent kinase 2; Kinase CDK2; p33 protein kinase; p33(CDK2); CCDS8898.1; ENSG00000123374
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK568
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T160
Protein UniProt: P24941
Protein SigNET: P24941
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDK2 sequence peptide Cat. No.: PE-04ADY95
Target Alias: Cyclin-dependent kinase 2; Kinase CDK2; p33 protein kinase; p33(CDK2); CCDS8898.1; ENSG00000123374
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK568
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T160
Protein UniProt: P24941
Protein SigNET: P24941
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDK2 sequence peptide Cat. No.: PE-04ADY95
Antibody Immunogen Sequence: RTY(pT)HEV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R157 to V163; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Immunogen Description: Corresponds to amino acid residues R157 to V163; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in Jurkat cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in Jurkat cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 33-37 kDa.
Antibody Specificity: Medium-High
Antibody Cross Reactivity: Medium to strong cross-reactivity with a ~78 kDa protein in HeLa cells, Jurkat cells, and sea star oocytes. The 78 kDa protein band shifts up with phorbol ester treatment of serum-starved HeLa and Jurkat cells or sea star oocyte maturation.
Related Product 1: CDK2-pT160 blocking peptide
Related Product 2: CDK1-3 Selectide - CDK1 (CDC2) protein kinase substrate peptide
Antibody Specificity: Medium-High
Antibody Cross Reactivity: Medium to strong cross-reactivity with a ~78 kDa protein in HeLa cells, Jurkat cells, and sea star oocytes. The 78 kDa protein band shifts up with phorbol ester treatment of serum-starved HeLa and Jurkat cells or sea star oocyte maturation.
Related Product 1: CDK2-pT160 blocking peptide
Related Product 2: CDK1-3 Selectide - CDK1 (CDC2) protein kinase substrate peptide
Scientific Background: CDK2 is a protein-serine/threonine kinase of the CMGC group and CDK family. It is essential for meiosis, yet not essential for mitosis, although it is involved in cell cycle control. CDK2 has been observed to phosphorylate CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, and EZH2. Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2. Mutations resulting in reduced CAK phosphorylation at the T160 activating phosphosite include K9F, and K88E + K89V, while a L166R mutation results in reduced CAK phosphorylation and lower protein-serine/threonine phosphotransferase activity. Phosphorylation at Y179 increases phosphotransferase activity and interaction with CCNA2 (Cyclin A2). CCNA2 stimulates the catalytic activity of CDK2. Phosphorylation at T14 and Y15 inhibits phosphotransferase activity.
Figure 1. Dot blotting CDK2-pT160 antibody with recombinant purified proteins.
Figure 2. Epitope mapping of CDK2-pT160 antibody with similar phosphopeptides on dot blots.
Figure 3. Identification of phosphosites related to CDK2-pT160.
© Kinexus Bioinformatics Corporation 2017