Product Name: CDC7-pT376
Product Number: AB-PK558
Size: 25 µg      Price:89.00
      $US
Target Full Name: Cell division cycle 7-related protein kinase

Target Alias: CDC7- related kinase; CDC7L1; Cell division cycle 7; HsCdc7; Hsk1; HuCdc7; Kinase CDC7; MuCdc7

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK558

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T376

Protein UniProt: O00311

Protein SigNET: O00311

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human CDC7 sequence peptide Cat. No.: PE-04AJE99
Antibody Immunogen Sequence: RAG(pT)PGF(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues R373 to F379; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Very weak immunoreactivity of a target-sized protein by Western blotting in sea star oocytes. Very strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 62-67 kDa.

Antibody Specificity: High-very high

Antibody Cross Reactivity: No significant cross-reactive proteins detected in in A431, HeLa and Jurkat cells.

Related Product 1: CDC7-pT376 blocking peptide

Scientific Background: CDC7 is a protein-serine/threonine kinase that is important for the G1/S transition in the cell division cycle. The protein product may phosphorylate critical substrates in G1/S phase transition and DNA replication. CDC7 can also phosphorylate MCM2 and MCM3. It is activated by phosphorylation at T376 in the kinase activation loop by CDK1 and CDK2. It also requires binding to activator of S-phase kinase (ASK)/DBF4B to be fully stimulated. It may be an oncoprotein (OP) as over-expression of CDC7 may be associated with neoplastic tumour transformation. RNAi inhibition of CDC7 induces an abortive S phase and apoptotic cell death in p53-mutant ErbB2-overexpressing and triple-negative (ER-/PR-/ErbB2-) breast cancer cell lines, but not in untransformed breast epithelial cells. Consequently, CDC7 appears to represent a potent and highly specific anticancer target in ErbB2-overexpressing and triple-negative breast cancers. It is also a suitable target for treatment of other types of cancer, including pancreatic cancer.
Figure 1. Epitope mapping of CDC7-pT376 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to CDC7-pT376.