Target Full Name: Calcium/calmodulin-dependent protein-serine kinase kinase

Target Alias: Calcium/calmodulin-dependent protein kinase 1 alpha, isoform a; Calcium/calmodulin-dependent protein kinase kinase 1, alpha; CaMKK alpha protein; CaMKK1.; CaMKKA; DKFZP761M0423; KKCC1; MGC34095

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK557

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: S74

Protein UniProt: Q8N5S9

Protein SigNET: Q8N5S9

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human CaMKK1 (CaMKK) sequence peptide Cat. No.: PE-04AJD99

Antibody Immunogen Sequence: RKL(pS)LQE(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues R71 to E77; In the N-terminal quarter of the protein located before the kinase catalytic domain. On the This is the major in vivo phosphorylation sites in CaMMK1.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.