Target Full Name: Calcium/calmodulin-dependent protein-serine kinase 2 alpha

Target Alias: Calcium/calmodulin-dependent protein kinase II alpha; Calcium/calmodulin-dependent protein kinase II alpha-B subunit; Calcium/calmodulin-dependent protein kinase type II alpha chain; CaM kinase II alpha subunit; CaMK2-alpha; CaMKA; CaMK-II alpha subunit; CaMKII-alpha; CaMKIINalpha; Kinase CaMK2-alpha;CaM-kinase II alpha chain; EC 2.7.11.17; KCC2A; KIAA0968

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK555

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T286

Protein UniProt: Q9UQM7

Protein SigNET: Q9UQM7

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human CaMK2a sequence peptide Cat. No.: PE-04AJB90

Antibody Immunogen Sequence: HRQE(pT)VDC

Antibody Immunogen Description: Corresponds to amino acid residues H282 to C289; In the region between the kinase catalytic domain and the CamKII-AD domains. The major site of in vivo phosphorylation of CaMK2a.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.