Product Name: BUB1B-pS670
Product Number: AB-PK552
Size: 25 µg      Price:89.00
      $US
Target Full Name: Mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Target Alias: BUB1 budding uninhibited by benzimidazoles 1 homologue beta (yeast); HBUBR1; Kinase BubR1; MAD3/BUB1-related protein kinase; MAD3L; SSK1; Bub1A; hBUBR1; BUB1beta; hBUBR1; CCDS10053.1; ENSG00000156970

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK552

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: S670

Protein UniProt: O60566

Protein SigNET: O60566

Antibody Type: Polyclonal

Antibody Host Species: Rabbit
Antibody Immunogen Source: Human BUB1B (Bub1R) sequence peptide Cat. No.: PE-04AIY99

Antibody Immunogen Sequence: KKL(pS)PII(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues K667 to I673; Just before kinase catalytic domain. This is the major in vivo phosphorylation sites.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in HeLa and T98G cells. Strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 115-130 kDa.

Antibody Specificity: High-very high

Antibody Cross Reactivity: No significant cross-reactive proteins detected in T98G cells, but a strong ~80 kDa protein was evident in HeLa cells, and a strong ~70 kDa protein was seen in sea star oocytes.

Related Product 1: BUB1B-pS670 blocking peptide

Scientific Background: BUBR1 (BUB1B) is a protein-serine/threonine kinase of the Other group and BUB family. It is an essential component of the mitotic checkpoint and is required for normal mitosis. It prevents progression of anaphase until all the sister chomatids are connected to spindles by binding to CDC20 to anaphase promoting complex (APC)/cycolsome. It is activated by the kinetochore motor protein CENPE, which increases phosphotransferase activity, and it is also involved in the localization of CENPE. BUBR1 negatively regulates Plk1 phosphotransferase activity in interphase cells, and it suppresses centrosome amplification and triggers apoptosis in polyploid cells that exit aberrantly from mitotic arrest. A K795R mutation inhibits the phosphotransferase activity of BUBR1, but does not abolish its ability to inhibit APC. BUBR1 may be a tumour suppressor protein (TSP). An R36Q mutation is linked with premature chromatid separation trait (PCS), which is an autosomal dominant disease. An R550Q mutation is found in Mosaic variegated aneuploidy syndrome 1 (MVA1), which is a severe developmental disease with characterized mosaic aneuploidies.
Figure 1. Epitope mapping of BUB1B-pS670 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to BUB1B-pS670.