Product Name: BRSK1-pT189
Product Number: AB-PK549
Size: 25 µg      Price:89.00
      $US
Target Full Name: BR serine/threonine-protein kinase 1

Target Alias: BR serine/threonine kinase 1; KIAA1811; SAD1; SAD1 kinase; SAD1A

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK549

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T189

Protein UniProt: Q8TDC3

Protein SigNET: Q8TDC3

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human BRSK1 sequence peptide Cat. No.: PE-04AIW95

Antibody Immunogen Sequence: LLE(pT)SCG(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues L186 to G192; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in HEK-293 cells. Very strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 85-90 kDa.

Antibody Specificity: Very high

Antibody Cross Reactivity: No cross-reactive proteins evident in Jurkat cells, HEK-293 cells and in sea star oocytes.

Related Product 1: BRSK1-pT189 blocking peptide

Related Product 2: BrskSelectide - BRSK1 protein kinase substrate peptide

Related Product 3: BrskSubtide - BRSK2 protein kinase substrate peptide

Scientific Background: BRSK1 is a protein-serine/threonine kinase of the CAMK group and CAMKL family. It is activated by phosphorylation on T205 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Its substrates include CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Its phosphotransferase activity is lost with the K59A, and G327A mutations. It is involved in the UV-induced DNA damage checkpoint response, likely by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C. A LKB1/LKB1 complex with the STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39 phosphorylates and activates BRSK1, but this can be prevented by an inhibitory T189A mutation.
Figure 1. Epitope mapping of BRSK1-pT189 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to BRSK1-pT189.