Product Name: BARK1-Y356
Product Number: AB-PK537
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Beta-adrenergic receptor kinase 1
Target Alias: ADRBK1; Adrenergic, beta, receptor kinase 1; ARBK1; BARK; BARK1; Beta-adrenergic receptor kinase 1; Beta-ARK-1; EC 2.7.11.15; FLJ16718; G- protein coupled receptor kinase 2; Kinase GRK2
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK537
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y356
Protein UniProt: P25098
Protein SigNET: P25098
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Target Alias: ADRBK1; Adrenergic, beta, receptor kinase 1; ARBK1; BARK; BARK1; Beta-adrenergic receptor kinase 1; Beta-ARK-1; EC 2.7.11.15; FLJ16718; G- protein coupled receptor kinase 2; Kinase GRK2
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK537
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y356
Protein UniProt: P25098
Protein SigNET: P25098
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human BARK1 (GRK2, ADRBK1) sequence peptide Cat. No.: PE-04ACJ85
Antibody Immunogen Sequence: THG(pY)MAP(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues T353 to P359; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Immunogen Sequence: THG(pY)MAP(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues T353 to P359; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity of a target-sized protein by Western blotting in FBS-treated Jurkat cells and weakly in HeLa cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity of a target-sized protein by Western blotting in FBS-treated Jurkat cells and weakly in HeLa cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 78-85 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No medium to strong cross-reactivities with other proteins in Jurkat cells, HeLa cells, and sea star oocytes.
Related Product 1: BARK1-Y356 blocking peptide
Related Product 2: BARK1-pS670 phosphosite-specific antibody (Cat. No.: AB-PK536)
Antibody Specificity: Very high
Antibody Cross Reactivity: No medium to strong cross-reactivities with other proteins in Jurkat cells, HeLa cells, and sea star oocytes.
Related Product 1: BARK1-Y356 blocking peptide
Related Product 2: BARK1-pS670 phosphosite-specific antibody (Cat. No.: AB-PK536)
Scientific Background: BARK1 (GRK2, ADRBK1) is a protein-serine/threonine kinase of the AGC group and GRK family. It is activated by G-beta/gamma subunit complex. Phosphorylation of S29 increases phosphotransferase activity. Phosphorylation of S670 inhibits G-beta/gamma-mediated activation of GRK2. Phosphorylation of Y13, Y86, Y92 and S685 increases interaction with G-alpha(q) and may induce protein degradation.
Figure 1. Epitope mapping of BARK1-Y356 antibody with similar phosphopeptides on dot blots.
Figure 2. Identification of phosphosites related to BARK1-Y356.
© Kinexus Bioinformatics Corporation 2017