Target Full Name: 5'-AMP-activated protein kinase catalytic subunit alpha-1

Target Alias: 5'-AMP-activated protein kinase, catalytic alpha-1 chain; AAPK1; Acetyl-CoA carboxylase kinase; AMPK alpha-1 chain; AMPK, alpha, 1; AMPK-alpha1; HMG-CoA reductase kinase; HMG-CoA reductase kinase; PRKAA1; Protein kinase, AMP-activated, alpha 1 catalytic subunit; MGC33776; MGC57364; CCDS3932.1; ENSG00000132356

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK521

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T183+S184

Protein UniProt: Q13131

Protein SigNET: Q13131

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human AMPKa1 (PRKAA1) sequence peptide Cat. No.: PE-04AIG99

Antibody Immunogen Sequence: FLR(pS)(pT)CG(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues F180 to G186; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.