Product Name: Akt1-pY315
Product Number: AB-PK516
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: RAC-alpha serine/threonine-protein kinase
Target Alias: Akt; Akt1; Akt1 kinase; C-Akt; Kinase Akt1; PKB; PKB-alpha; PRKBA; V-akt murine thymoma viral oncogene 1; Protein kinase B; RAC; RAC-alpha serine,threonine kinase; RAC-alpha serine/threonine kinase; RAC-PK-alpha; PKBA; hCG_96740; Akt1_NEW; Akt; MGC99656; CCDS9994; B0LPE5; B2RAM5; B3KVH4; B3KXD7; B7Z5R1; Q9BV07; ENSG00000142208
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK516
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y315
Protein UniProt: P31749
Protein SigNET: P31749
Target Alias: Akt; Akt1; Akt1 kinase; C-Akt; Kinase Akt1; PKB; PKB-alpha; PRKBA; V-akt murine thymoma viral oncogene 1; Protein kinase B; RAC; RAC-alpha serine,threonine kinase; RAC-alpha serine/threonine kinase; RAC-PK-alpha; PKBA; hCG_96740; Akt1_NEW; Akt; MGC99656; CCDS9994; B0LPE5; B2RAM5; B3KVH4; B3KXD7; B7Z5R1; Q9BV07; ENSG00000142208
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK516
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y315
Protein UniProt: P31749
Protein SigNET: P31749
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Akt1 (PKBa) sequence peptide Cat. No.: PE-04ACH99
Antibody Immunogen Sequence: TPE(pY)LAP(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues T312 to P318; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Akt1 (PKBa) sequence peptide Cat. No.: PE-04ACH99
Antibody Immunogen Sequence: TPE(pY)LAP(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues T312 to P318; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots. Very strong immunoreactivity with recombinant human Akt1 on protein dot blots.
Antibody Species Reactivity: Human
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots. Very strong immunoreactivity with recombinant human Akt1 on protein dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 57-62 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: Strong immunoreactivity on protein dot blots with Akt2 and Akt3, which feature identical phosphosite sequences. No cross-reactive proteins detected in HepG2 cells, MCF7 cells or seastar oocytes.
Related Product 1: Akt1-pY315 blocking peptide
Related Product 2: Akt1-1 pan-specific antibody (Cat. No.: AB-NK129-3)
Related Product 3: Akt1-2 pan-specific antibody (Cat. No.: AB-NK129-4)
Related Product 4: Akt1-3 pan-specific antibody (Cat. No.: AB-NK129-5)
Antibody Specificity: Very high
Antibody Cross Reactivity: Strong immunoreactivity on protein dot blots with Akt2 and Akt3, which feature identical phosphosite sequences. No cross-reactive proteins detected in HepG2 cells, MCF7 cells or seastar oocytes.
Related Product 1: Akt1-pY315 blocking peptide
Related Product 2: Akt1-1 pan-specific antibody (Cat. No.: AB-NK129-3)
Related Product 3: Akt1-2 pan-specific antibody (Cat. No.: AB-NK129-4)
Related Product 4: Akt1-3 pan-specific antibody (Cat. No.: AB-NK129-5)
Related Product 5: Akt1-pS473 phosphosite-specific antibody (Cat. No.: AB-PK869)
Related Product 6: Akt1-pS473+pY474 phosphosite-specific antibody (Cat. No.: AB-PK514)
Related Product 7: Akt1-pT308 phosphosite-specific antibody (Cat. No.: AB-PK515)
Related Product 8: Akt1-pY326 phosphosite-specific antibody (Cat. No.: AB-PK517)
Related Product 9: AktSubtide - Akt1 (PKBa) protein kinase substrate peptide
Related Product 10: GSK3b (3-12) KinSub - Glycogen synthase kinase 3-beta (GSK3b) N-terminus (G3-E12, human) peptide; Akt protein kinase substrate peptide
Related Product 6: Akt1-pS473+pY474 phosphosite-specific antibody (Cat. No.: AB-PK514)
Related Product 7: Akt1-pT308 phosphosite-specific antibody (Cat. No.: AB-PK515)
Related Product 8: Akt1-pY326 phosphosite-specific antibody (Cat. No.: AB-PK517)
Related Product 9: AktSubtide - Akt1 (PKBa) protein kinase substrate peptide
Related Product 10: GSK3b (3-12) KinSub - Glycogen synthase kinase 3-beta (GSK3b) N-terminus (G3-E12, human) peptide; Akt protein kinase substrate peptide
Scientific Background: Akt1 (PKBA) is a protein-serine/threonine kinase of the AGC group and Akt family. It is normally inactive in the cytoplasm. When PI3K is activated by extracellular growth factors, Akt1 is recruited to the cell membrane and phosphorylated by PDK1, mTOR and other protein kinases to become active. This leads to the induction of survival, proliferation, angiogenesis and metabolism pathways. Akt1 is also involved in cell-cycle progression via its inhibitory phosphorylation of cyclin-dependent kinases and activatory phosphorylation of mTOR. This kinase is highly expressed in most tested human tissues. Orthologues of Akt are conserved in a wide range of animals, plants and unicellular eukaryotes. Phosphorylation of T72, S124,S129, S246, T308, Y315,Y326, T450, S473 and Y474 increases Akt1 phosphotransferase activity. TRAF6-induced 'K63'-linked Akt1 ubiquitination is critical for phosphorylation and activation. Phosphorylation of T34 inhibits phosphotransferase activity. Akt1 has been implicated in a wide range of human cancers, including lymphomas, breast, lung, colorectal, pancreatic and prostate cancers.
Figure 1. Epitope mapping of Akt1-pY315 antibody with similar phosphopeptides on dot blots.
Figure 2. Identification of phosphosites related to Akt1-pY315.
© Kinexus Bioinformatics Corporation 2017