Product Name: Abl2-pY439
Product Number: AB-PK509
Size: 25 µg      Price:89.00
      $US
Target Full Name: Abelson murine leukemia viral oncogene homologue 2

Target Alias: Abelson-related gene; ABLL; ARG; Kinase Arg; Tyrosine kinase ARG; v-Abl Abelson murine leukemia viral oncogene 2; v-Abl Abelson murine leukemia viral oncogene homologue 2; hCG_23565; FLJ22224; FLJ31718; FLJ41441; RP11-177A2_3; ENSG00000143322; D1MPS6

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK509

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y439

Protein UniProt: P42684

Protein SigNET: P42684

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human Abl2 (Arg) sequence peptide Cat. No.: PE-04AII85

Antibody Immunogen Sequence: GDT(pY)TAH(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues G436 to H442; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.4 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 130-140 kDa.

Antibody Specificity: Very high

Antibody Cross Reactivity: No cross-reactive proteins detected in Jurkat cells.

Related Product 1: Abl2-pY439 blocking peptide

Related Product 2: Abl2-1 pan-specific antibody (Cat. No.: AB-NK238-1)

Related Product 3: Abl2-pY439+pT440 phosphosite-specific antibody (Cat. No.: AB-PK510)

Related Product 4: AblSubtide - Abl1 protein kinase substrate peptide

Scientific Background: Arg (Abl2) is a protein-tyrosine kinase of the TK group and Abl family. It features SH3 and SH2 domains, a protein- tyrosine kinase domain, and a nuclear localization domain. It shares many functions with Abl1, which includes cytoskeleton remodelling through phosphorylation of enzymes responsible for cytoskeleton rearrangements for signalling, adhesion, and movement as well as microtuble subunits, TUBA1 and TUBB. It also contains F-actin binding region to bundle and regulate actin cytoskeletal structure. It possesses autocatalytic activity and the ability to phosphorylate its inhibitor ABI1 to regulate itself. Arg gene expression is found in both normal and tumour cells, it is often found as a fusion gene with ETV6 in acute non lymphocytic leukemias. However, the broad distribution of point mutations seen in various cancers, including about a third within the kinase catalytic domain, indicate that these mutations are likely to be silent or result in loss of function. Arg has also been reported to promote breast tumour cell invasion and dissemination, but attenuates the growth of breast tumours. This indicates that wild-type Arg may as a tumour suppressor protein. Phosphorylation of Tyr-261 reduces ubiquitination and protein degradation. Arg is activated by autophosphorylation as well as by Src-family kinase-mediated phosphorylation. It is also activated by RIN1 binding to the SH2 and SH3 domains.
Figure 1. Epitope mapping of Abl2-pY439 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to Abl2-pY439.






















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