Product Name: A6r-pY309
Product Number: AB-PK502
Size: 25 µg      Price:89.00
      $US
Target Full Name: Twinfilin, actin-binding protein, 2; Protein tyrosine kinase 9-like

Target Alias: TWF2; PTK9L

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK502

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y309

Protein UniProt: Q6IBS0

Protein SigNET: Q6IBS0

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human A6r (Twinfin-2) sequence peptide Cat. No.: PE-04ACE99
Antibody Immunogen Sequence: EFL(pY)DEV(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues E306 to V312; In the end of the Cofilin_ADF domain. The major site of phosphorylation of A6r.

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 39-40 kDa.

Antibody Cross Reactivity: No significant cross-reactive proteins detected in seastar oocytes.

Related Product 1: A6r-pY309 blocking peptide

Related Product 2: A6-pY309 phosphosite-specific antibody (Cat. No.: AB-PK501)

Scientific Background: A6r appears to be an actin-binding protein involved in motile and morphological processes. It inhibits actin polymerization, probably by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. It seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles.
Figure 1. Epitope mapping of A6r-pY309 antibody with similar phosphopeptides on dot blots.


Figure 2. Identification of phosphosites related to A6r-pY309.