Product Name: PKR1-pT446
Product Number: AB-PK132
Size: 25 µg      Price:89.00
      $US
Target Full Name: Protein-serine/threonine kinase Interferon-induced, double-stranded RNA-activated protein kinase Thr-446 phosphosite

Target Alias: ADRB2; E2AK2; EC 2.7.11.1; EIF2aK; EIF2AK1; Eukaryotic translation initiation factor 2-alpha kinase 2; Interferon-inducible RNA-dependent protein kinase; Kinase PKR; P1,eIF-2A protein kinase; P1/eIF-2A protein kinase; P68 kinase; PRKR; TIK

Product Type Specific: Phosphosite-specific polyclonal antibody for human PKR T446 phosphosite

Antibody Code: PK132

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T446

Protein UniProt: P19525

Protein SigNET: P19525
Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Ig Isotype Clone: IgG

Antibody Immunogen Source: Peptide with amino acid sequence surrounding the human PKR Thr-446 phosphosite (Lys-Arg-pThr-Arg-Ser)

Antibody Immunogen Sequence: KRpTRS

Antibody Immunogen Description: 5 amino acid phosphopeptide corresponding to the human PKR1 T446 phosphosite

Production Method: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol

Storage Conditions: -20°C

Storage Stability: > 1 year

Product Use: Western blotting, Immunohistochemistry (IHC)

Antibody Dilution Recommended: 2 µg/mL

Antibody Potency: Very strong

Antibody Species Reactivity: Human, Mouse

Antibody Positive Control: T446 phosphorylated PKR1

Antibody Specificity: Medium

Antibody Cross Reactivity: Cross-reactivities observed with ~16, ~42, ~60 and ~120 kDa proteins.
Related Product 1: PKR1-pT446 phospho-specific antibody (Cat. No.: AB-PK132)

Related Product 2: PKR1-pT446 phospho-specific antibody (Cat. No.: AB-PK777)

Scientific Background: PKR a protein kinase of the PEK family. Upon binding double-stranded RNA (which can arise from certain viral infections) or heparin, it autophosphorylates at Thr-451 and become activated. It is also activated by interaction with HIV-1 Tat. PKR phosphorylates and inhibits the alpha subunit of eIF2 alpha (EIF2S1), which leads to an inhibition of the initiation of protein synthesis. It controls the activation of several transcription factors such as NF-kappaB, p53 and Stats. PKR mediates apoptosis induced by many different stimuli, such as lipopolysaccharide (LPS), TNF-alpha, viral infection and serum starvation.
Figure 1. Immunoblotting of various cell lines with AB-PK132 antibody at a 500 X dilution. The target protein PKR1 is indicated. Each lane was loaded with 15 µg of cell lysate protein. The max signal count was 13530.


Figure 2. Immunoblotting of various cell lines with AB-PK126 antibody at a 500 X dilution. The target protein PKR1 is indicated. Each lane was loaded with 15 µg of cell lysate protein. The max signal count was 56198.


References

[1] Zhang F, Romano PR, Nagamura-Inoue T, Tian B, Dever TE, Mathews MB, Ozato K, Hinnebusch AG. Binding of double-stranded RNA to protein kinase PKR is required for dimerization and promotes critical autophosphorylation events in the activation loop. J Biol Chem. 2001 Jul 6;276(27):24946-58. Epub 2001 May 3. PMID: 11337501.