Product Name: JAK1-pY1022
Product Number: AB-PK126
Size: 25 µg      Price:89.00
      $US
Target Full Name: Protein-tyrosine kinase JAK1 Tyr-1022 phosphosite

Target Alias: EC 2.7.10.2; JAK1A; Janus kinase 1

Product Type Specific: Phosphosite-specific polyclonal antibody for human JAK1 Y1022 phosphosite

Antibody Code: PK126

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: Y1022

Protein UniProt: P23458

Protein SigNET: P23458

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Ig Isotype Clone: IgG

Antibody Immunogen Source: Peptide with amino acid sequence surrounding the human JAK1 Tyr-1022 phosphosite (Lys-Glu-pTyr-Tyr-Thr)

Antibody Immunogen Sequence: KEpYYT

Antibody Immunogen Description: 5 amino acid phosphopeptide corresponding to the human JAK1 Y1022 phosphosite

Production Method: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol

Storage Conditions: -20°C
Storage Stability: > 1 year

Product Use: Western blotting, Immunohistochemistry (IHC)

Antibody Dilution Recommended: 2 µg/mL

Antibody Potency: Strong

Antibody Species Reactivity: Human, Mouse, Rat

Antibody Positive Control: Y1022 phosphorylated JAK1

Antibody Specificity: High

Antibody Cross Reactivity: Ocassionally, strong cross-reactivity with a ~23 kDa protein and weaker cross-reactivity with a ~50 kDa protein can be detected.

Related Product 1: JAK1-1 pan-specific antibody (Cat. No.: AB-NK084-3)

Related Product 2: JAK1-3 pan-specific antibody (Cat. No.: AB-NK084-5)
Scientific Background: JAK1 is a widely expressed non-receptor tyrosine-kinase involved in the interferon-alpha/beta and -gamma signal transduction pathways, and can bind directly to the Interleukin 2 receptor. It couples cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and of a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs. It is known to interact with IL31RA, IFNAR2, JAKMIP1 (via FERM domain) and SHB. It is expressed at higher levels in primary colon tumors than in normal colon tissue, but its expression level in metastatic colon tumors is comparable to the level in normal colon tissue. It is also activated in B cell lines from patients with post-transplant lymphoproliferative disorder, and in a mouse Lck-driven T cell lymphoma. JAK family kinases feature two protein kinase domains. Piceatannol is a known JAK1 inhibitor.
Figure 1. Immunoblotting of various cell lines with AB-PK126 antibody at a 500 X dilution. The target protein JAK1 is indicated. Each lane was loaded with 15 µg of cell lysate protein. The max signal count was 56429.


Figure 2. Immunoblotting of various cell lines with AB-PK126 antibody at a 500 X dilution. The target protein JAK1 is indicated. Each lane was loaded with 15 µg of cell lysate protein. The max signal count was 28833.


References

[1] Zheng H, Hu P, Quinn DF, Wang YK. Phosphotyrosine proteomic study of interferon alpha signaling pathway using a combination of immunoprecipitation and immobilized metal affinity chromatography. Mol Cell Proteomics. 2005 Jun;4(6):721-30. Epub 2005 Jan 19. PMID: 15659558.

[2] Wang R, Griffin PR, Small EC, Thompson JE. Mechanism of Janus kinase 3-catalyzed phosphorylation of a Janus kinase 1 activation loop peptide. Arch Biochem Biophys. 2003 Feb 1;410(1):7-15. PMID: 2559972.