Product Name: Chk2-pT68
Product Number: AB-PK119
Size: 25 µg      Price:89.00
      $US
Target Full Name: Protein-serine/threonine kinase Chk2 Thr-68 phosphosite

Target Alias: Cds1; CHEK2; CHK2 checkpoint homologue (S. pombe); EC 2.7.11.1; RAD53

Product Type Specific: Phosphosite-specific polyclonal antibody for human Chk2 T68 phosphosite

Antibody Code: PK119

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T68

Protein UniProt: O96017

Protein SigNET: O96017

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Ig Isotype Clone: IgG
Antibody Immunogen Source: Peptide with amino acid sequence surrounding the human Chk2 Thr-68 phosphosite (Val-Ser-pThr-Gln-Glu)

Antibody Immunogen Sequence: VSpTQE

Antibody Immunogen Description: 5 amino acid phosphopeptide corresponding to the human Chk2 T68 phosphosite

Production Method: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol

Storage Conditions: -20°C
Storage Stability: > 1 year

Product Use: Western blotting, Immunohistochemistry (IHC)

Antibody Dilution Recommended: 2 µg/mL

Antibody Potency: Very strong

Antibody Species Reactivity: Human

Antibody Positive Control: T68 phosphorylated Chk2

Antibody Specificity: High

Antibody Cross Reactivity: Ocassionally, strong cross-reactivities with ~46 and ~100 kDa proteins can be detected.

Related Product 1: Chk2-pT68 phosphosite-specific antibody (Cat. No.: AB-PK581)

Related Product 2: Chk2-pT383 phosphosite-specific antibody (Cat. No.: AB-PK580)

Related Product 4: Chk2Selectide - Chk2 (CHEK2) protein kinase substrate peptide

Scientific Background: Chk2 is a CAMK protein kinase of the Rad53 family that acts as a cell cycle checkpoint regulator and putative tumour suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage such as DNA double-strand breaks. It is rapidly phosphorylated at Thr-68 by MLTK in response to replication blocks and DNA damage, and activated through autophosphorylation. Chk2 phosphorylates CDC25C at Ser-216 and inhibits this phosphatase (blocking entry into mitosis), p53 at Thr-18 and Ser-20 (inducing cell cycle arrest in G1), and BRCA1 (maintaining survival after DNA damage). Twelve splice variant isoforms have been described for human Chk2. High expression is observed in testis, spleen, colon and peripheral blood leukocytes. Loss of function mutants of ChK2 can cause Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype often found with p53 mutations as well. Familial mutations also associated with prostate and breast cancers, and mutations also occur in a variety of sporadic cancers and cell lines.
Figure 1. Immunoblotting of various cell lines with AB-PK119 antibody at a 500 X dilution. The target protein Chk2 is indicated. Each lane was loaded with 15 µg of cell lysate protein. The max signal count was 37307.


Figure 2. Immunoblotting of various cell lines with AB-PK119 antibody at a 500 X dilution. The target protein Chk2 is indicated. Each lane was loaded with 15 µg of cell lysate protein. The max signal count was 40455.


References

[1] Matsuoka S, Rotman G, Ogawa A, Shiloh Y, Tamai K, Elledge SJ. Ataxia telangiectasia-mutated phosphorylates Chk2 in vivo and in vitro. Proc Natl Acad Sci U S A. 2000 Sep 12;97(19):10389-94. PMID: 10973490

[2] Gorgoulis VG, Vassiliou LV, Karakaidos P, Zacharatos P, Kotsinas A, Liloglou T, Venere M, Ditullio RA Jr, Kastrinakis NG, Levy B, Kletsas D, Yoneta A, Herlyn M, Kittas C, Halazonetis TD. Activation of the DNA damage checkpoint and genomic instability in human precancerous lesions. Nature. 2005 Apr 14;434(7035):907-13. PMID: 15829965.

[3] Falck J, Coates J, Jackson SP. Conserved modes of recruitment of ATM, ATR and DNA-PKcs to sites of DNA damage. Nature. 2005 Mar 31;434(7033):605-11. PMID: 15758953.

[4] Jin ZH, Kurosu T, Yamaguchi M, Arai A, Miura O. Hematopoietic cytokines enhance Chk1-dependent G2/M checkpoint activation by etoposide through the Akt/GSK3 pathway to inhibit apoptosis. Oncogene. 2005 Mar 17;24(12):1973-81.PMID: 15674326.

[5] Li J, Stern DF. Regulation of CHK2 by DNA-dependent protein kinase. J Biol Chem. 2005 Mar 25;280(12):12041-50. PMID: 15668230.