Product Name: Calcineurin A
Product Number: AB-NP049-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
Target Alias: Alpha isoform formerly PPP2B; Calcineurin A alpha; Calcineurin A1; CalcineurinA; Calmodulin dependent calcineurin A subunit alpha isoform; Calmodulin-dependent calcineurin A subunit alpha isoform; CALN; CALNA; CALNA 1; CAM PRP catalytic subunit; CCN 1; Protein phosphatase 2B catalytic subunitProtein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform; Protein phosphatase 3 catalytic subunit alpha isoform PPP3CA; Protein phosphatase 3 catalytic subunit alpha isozyme
Product Type Specific: Protein phosphatase pan-specific antibody
Antibody Code: NP049-1
Antibody Target Type: Pan-specific
Protein UniProt: Q08209
Target Alias: Alpha isoform formerly PPP2B; Calcineurin A alpha; Calcineurin A1; CalcineurinA; Calmodulin dependent calcineurin A subunit alpha isoform; Calmodulin-dependent calcineurin A subunit alpha isoform; CALN; CALNA; CALNA 1; CAM PRP catalytic subunit; CCN 1; Protein phosphatase 2B catalytic subunitProtein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform; Protein phosphatase 3 catalytic subunit alpha isoform PPP3CA; Protein phosphatase 3 catalytic subunit alpha isozyme
Product Type Specific: Protein phosphatase pan-specific antibody
Antibody Code: NP049-1
Antibody Target Type: Pan-specific
Protein UniProt: Q08209
Protein SigNET: Q08209
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Human Calcineurin A peptide (AA 264-283)
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4; 50% glycerol, 0.09% azide
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Human Calcineurin A peptide (AA 264-283)
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4; 50% glycerol, 0.09% azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:120), IHC (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: High potency. Detects a ~61 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat | Dog | Rabbit
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:120), IHC (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: High potency. Detects a ~61 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat | Dog | Rabbit
Antibody Positive Control: 1 µg/ml of SPC-175 was sufficient for detection of Calcineurin A in 20 µg of Heat shock Hela lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: High
Antibody Specificity: High
Scientific Background: Calcineurin is a heterodimeric phosphatase protein, also known as calcium-dependent serine-threonine phosphatase. The structure consists of a catalytic subunit alpha, Calcineurin A (57-59kDa) the active site and a Ca2+ binding unit, Calcineurin B (19-20kDa) the regulatory subunit(1). Calcineurin plays a key role in the T-cell response growth and differentiation mechanism, regulating the activation of the Nuclear factor of activated T-cells (NFATc) which are important in the expression of IL-2 genes. Calcineurin has been the target of inhibitors, the novel and structural immune-suppressants antifungal drugs(2). Genetic studies in yeast and fungi established the molecular basis of the inhibition mechanism by cyclosporine A and FK506 (3).
References
[1] Rusnak F. and Mertz P. (2000) Physiol Rev. 80(4):1483-521.
[2] Hemenway C.S. And Heitman J. (1999)Cell Biochem Biophys. 30(1):115-51.
[3] Fox D.S. and Heitman J. (2002) Bioessays. 24(10):894-903.
[1] Rusnak F. and Mertz P. (2000) Physiol Rev. 80(4):1483-521.
[2] Hemenway C.S. And Heitman J. (1999)Cell Biochem Biophys. 30(1):115-51.
[3] Fox D.S. and Heitman J. (2002) Bioessays. 24(10):894-903.
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