Product Name: SHIP2-1
Product Number: AB-NP045-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 2
Target Alias: 51C protein; Inositol polyphosphate 5-phosphatase; Inositol polyphosphate phosphatase-like 1; SH2-containing inositol 5-phosphatase 2; SHIP2
Product Type Specific: SHIP2 (INPPL1) pan-specific antibody
Antibody Code: NP045-1
Antibody Target Type: Pan-specific
Protein UniProt: O15357
Protein SigNET: O15357
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human SHIP2 (INPPL1) sequence peptide Cat. No.: PE-01BBS90
Target Alias: 51C protein; Inositol polyphosphate 5-phosphatase; Inositol polyphosphate phosphatase-like 1; SH2-containing inositol 5-phosphatase 2; SHIP2
Product Type Specific: SHIP2 (INPPL1) pan-specific antibody
Antibody Code: NP045-1
Antibody Target Type: Pan-specific
Protein UniProt: O15357
Protein SigNET: O15357
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human SHIP2 (INPPL1) sequence peptide Cat. No.: PE-01BBS90
Antibody Immunogen Sequence: CGDREWLDLLRGGLKE
Antibody Immunogen Description: Corresponds to amino acid residues G477 to E491
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Immunogen Description: Corresponds to amino acid residues G477 to E491
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 130-160 kDa.
Related Product 1: SHIP2-1 blocking peptide
Related Product 2: SHIP2-2 pan-specific antibody (Cat. No.: AB-NP045-2)
Related Product 3: SHIP2-3 pan-specific antibody (Cat. No.: AB-NP045-3)
Related Product 4: SHIP2-pY886 phosphosite-specific antibody (Cat. No.: AB-PN534)
Related Product 1: SHIP2-1 blocking peptide
Related Product 2: SHIP2-2 pan-specific antibody (Cat. No.: AB-NP045-2)
Related Product 3: SHIP2-3 pan-specific antibody (Cat. No.: AB-NP045-3)
Related Product 4: SHIP2-pY886 phosphosite-specific antibody (Cat. No.: AB-PN534)
Scientific Background: SHIP2 (INPPL1) is a phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, and negatively regulate the PI3K (phosphoinositide 3-kinase) pathways. While overexpression reduces both insulin-stimulated MAP kinase and Akt activation, its absence does not affect insulin signalling or GLUT4 trafficking. It confers resistance to dietary obesity. It may also hydrolyze PtdIns(1,3,4,5)P4.
© Kinexus Bioinformatics Corporation 2017