Product Name: DUSP7-3
Product Number: AB-NP041-3
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Dual specificity protein phosphatase 7
Target Alias: Dual specificity protein phosphatase PYST2; DUS7; DUS7_HUMAN; PYST2
Product Type Specific: Protein phosphatase pan-specific antibody
Antibody Code: NP041-3
Antibody Target Type: Pan-specific
Protein UniProt: Q16829
Protein SigNET: Q16829
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human DUSP7 sequence peptide Cat. No.: PE-01BBF99
Antibody Immunogen Sequence: CPTNHNLFPLNTLEST
Target Alias: Dual specificity protein phosphatase PYST2; DUS7; DUS7_HUMAN; PYST2
Product Type Specific: Protein phosphatase pan-specific antibody
Antibody Code: NP041-3
Antibody Target Type: Pan-specific
Protein UniProt: Q16829
Protein SigNET: Q16829
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human DUSP7 sequence peptide Cat. No.: PE-01BBF99
Antibody Immunogen Sequence: CPTNHNLFPLNTLEST
Antibody Immunogen Description: Corresponds to amino acid residues P405 to T409; C-terminus
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human; Mouse
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 37-43 kDa.
Antibody Specificity: High
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human; Mouse
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 37-43 kDa.
Antibody Specificity: High
Related Product 1: DUSP7-3 blocking peptide
Related Product 2: DUSP7-1 pan-specific antibody (Cat. No.: AB-NP041-1)
Related Product 3: DUSP7-2 pan-specific antibody (Cat. No.: AB-NP041-2)
Related Product 2: DUSP7-1 pan-specific antibody (Cat. No.: AB-NP041-1)
Related Product 3: DUSP7-2 pan-specific antibody (Cat. No.: AB-NP041-2)
Scientific Background: DUSP7 (PYST2) is a dual specificity phosphatase that dephosphorylates MAP kinases on phospho-tyrosine and phospho- threonine residues and negatively regulates their phosphotransferase activity in response to changes in the cellular environment. PYST2-S may act as a negative regulator of PYST2-L, although it is unclear whether this is by competing for transcription, translation or activation factors.
© Kinexus Bioinformatics Corporation 2017