Product Name: DUSP1-2 (MKP-1)
Product Number: AB-NP006-2
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: MAP kinase phosphatase 1 (CL100, VH1)
Target Alias: 3CH134; CL100; DUS1; DUSP1; ERP; HVH1; PTPN10; PTPN16
Product Type Specific: Protein phosphatase pan-specific antibody
Antibody Code: NP006-2
Antibody Target Type: Pan-specific
Protein UniProt: P28562
Protein SigNET: P28562
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human DUSP1 (MKP1) sequence peptide Cat. No.: PE-01ATA99
Antibody Immunogen Sequence: CGAMGLEHIVPNAEL
Target Alias: 3CH134; CL100; DUS1; DUSP1; ERP; HVH1; PTPN10; PTPN16
Product Type Specific: Protein phosphatase pan-specific antibody
Antibody Code: NP006-2
Antibody Target Type: Pan-specific
Protein UniProt: P28562
Protein SigNET: P28562
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human DUSP1 (MKP1) sequence peptide Cat. No.: PE-01ATA99
Antibody Immunogen Sequence: CGAMGLEHIVPNAEL
Antibody Immunogen Description: Corresponds to amino acid residues G58 to L71
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Species Reactivity: Human; Mouse
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 39-44 kDa.
Antibody Specificity: Medium
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Species Reactivity: Human; Mouse
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 39-44 kDa.
Antibody Specificity: Medium
Related Product 1: DUSP1-2 (MKP-1) blocking peptide
Related Product 2: DUSP1-1 (MKP-1) pan-specific antibody (Cat. No.: AB-NP006-4)
Related Product 3: DUSP1-3 (MKP-1) pan-specific antibody (Cat. No.: AB-NP006-3)
Related Product 4: DUSP1 (MKP-1) pan-specific antibody (Cat. No.: AB-NP006-2P)
Related Product 2: DUSP1-1 (MKP-1) pan-specific antibody (Cat. No.: AB-NP006-4)
Related Product 3: DUSP1-3 (MKP-1) pan-specific antibody (Cat. No.: AB-NP006-3)
Related Product 4: DUSP1 (MKP-1) pan-specific antibody (Cat. No.: AB-NP006-2P)
Scientific Background: DUSP1 (MKP1) is a dual specificity phosphatase that dephosphorylates MAP kinase ERK2 (and ERK1) on both 'T183' and 'Y185', and inhibits its activity during the meiotic cell cycle.
Figure 1. Immunoblotting of various cell lines with NP006-2 antibody at a 500 X dilution. The target protein DUSP1 is indicated. The expected molecular mass of DUSP1 is 39,298 Da. Each lane was loaded with 20 µg of cell lysate protein.
© Kinexus Bioinformatics Corporation 2017