Product Name: LAR/PTPRF
Product Number: AB-NP005-2
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Receptor-type tyrosine-protein phosphatase F
Target Alias: FLJ43335; FLJ45062; FLJ45567; LAR; LAR protein; LARFN5C; LARS; LCA homologue; Leukocyte antigen related (LAR) PTP receptor; Leukocyte antigen related; Leukocyte antigen related PTP receptor; Leukocyte antigen related tyrosine phosphatase; Leukocyte common antigen related; Protein Tyrosine Phosphatase Receptor Type F; Protein tyrosine phosphatase receptor type F polypeptide; PTPRF protein; Receptor linked phosphatase LAR;
Product Type Specific: Protein phosphatase pan-specific antibody
Antibody Code: NP005-2
Antibody Target Type: Pan-specific
Protein UniProt: P10586
Protein SigNET: P10586
Antibody Type: Monoclonal
Target Alias: FLJ43335; FLJ45062; FLJ45567; LAR; LAR protein; LARFN5C; LARS; LCA homologue; Leukocyte antigen related (LAR) PTP receptor; Leukocyte antigen related; Leukocyte antigen related PTP receptor; Leukocyte antigen related tyrosine phosphatase; Leukocyte common antigen related; Protein Tyrosine Phosphatase Receptor Type F; Protein tyrosine phosphatase receptor type F polypeptide; PTPRF protein; Receptor linked phosphatase LAR;
Product Type Specific: Protein phosphatase pan-specific antibody
Antibody Code: NP005-2
Antibody Target Type: Pan-specific
Protein UniProt: P10586
Protein SigNET: P10586
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2a
Antibody Immunogen Source: Fusion protein amino acids 1315-1607 (cytoplasmic C-terminus) of human LAR. 97% identical in both rat and mouse. >80% identity with PTPRD and PTPRS. >50% identity with PTPRM and PTPRK.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.1% sodium azide
Antibody Ig Isotype Clone: IgG2a
Antibody Immunogen Source: Fusion protein amino acids 1315-1607 (cytoplasmic C-terminus) of human LAR. 97% identical in both rat and mouse. >80% identity with PTPRD and PTPRS. >50% identity with PTPRM and PTPRK.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~85 kDa protein (full length protein is 210 kDa protein – smaller due to proteolysis into P-subunit containing transmembrane and intracellular domains.
Antibody Species Reactivity: Human | Mouse | Rat
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~85 kDa protein (full length protein is 210 kDa protein – smaller due to proteolysis into P-subunit containing transmembrane and intracellular domains.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-443 was sufficient for detection of LAR/PTPRF in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Scientific Background: PTPRF or leukocyte common antigen-related protein (LAR) is a widely expressed protein tyrosine phosphatase with an extracellular receptor region that resembles a cell adhesion molecule. PTPRF removes phosphate group from β-catenin, an event that may subsequently facilitate cell-cell adhesion and ensure the stability of the cadherin complex. This phosphatase has also been implicated in various cellular processes such as neurite growth, nerve regeneration, actin remodeling and regulation of insulin function (1,2,3,4). Anti-PTPRF (C-terminal) antibody is specific for the extracellular and cytoplasmic subunits of human PTPRF (approx. 210, 150 and 85 kDa). Detection of the PTPRF bands by immunoblotting is specifically inhibited by the immunizing peptide.
References
[1] Streuli M. M. et al. (1992) EMBO Journal, 11(3), 897-907.
[2] Mooney R.A. and C.M LeVea. (2003) Current Topics in Medicinal Chemistry, 3(7), 809-819.
[3] Chagnon M.J. et al. (2004) Biochemistry and Cell Biology, 82(6), 664-675.
[4] Ahmad F.F., and B.J. Goldstein (1997) Journal of Biological Chemistry, 272(1), 448-457.
[1] Streuli M. M. et al. (1992) EMBO Journal, 11(3), 897-907.
[2] Mooney R.A. and C.M LeVea. (2003) Current Topics in Medicinal Chemistry, 3(7), 809-819.
[3] Chagnon M.J. et al. (2004) Biochemistry and Cell Biology, 82(6), 664-675.
[4] Ahmad F.F., and B.J. Goldstein (1997) Journal of Biological Chemistry, 272(1), 448-457.
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