Product Name: CDC25C-3
Product Number: AB-NP003-3
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Cell division cycle 25C phosphatase
Target Alias: CDC25; CDC25M1; Cell division cycle 25 C; M-phase inducer phosphatase 3; MPI1B; MPIP3
Product Type Specific: Protein phosphatase pan-specific antibody
Antibody Code: NP003-3
Antibody Target Type: Pan-specific
Protein UniProt: P30307
Protein SigNET: P30307
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDC25C sequence peptide Cat. No.: PE-01AZS95
Antibody Immunogen Sequence: CRQLREQIALLVKDMS
Target Alias: CDC25; CDC25M1; Cell division cycle 25 C; M-phase inducer phosphatase 3; MPI1B; MPIP3
Product Type Specific: Protein phosphatase pan-specific antibody
Antibody Code: NP003-3
Antibody Target Type: Pan-specific
Protein UniProt: P30307
Protein SigNET: P30307
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDC25C sequence peptide Cat. No.: PE-01AZS95
Antibody Immunogen Sequence: CRQLREQIALLVKDMS
Antibody Immunogen Description: Corresponds to amino acid residues R458 to S472
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with recombinant human CDC25C on protein dot blots.
Antibody Species Reactivity: Human; Mouse
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with recombinant human CDC25C on protein dot blots.
Antibody Species Reactivity: Human; Mouse
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 60-65 kDa.
Antibody Cross Reactivity: Weak immunoreactivity on protein dot blots with recombinant human CDC25A, and very weak immunoreactivity with recombinant human CDC25B.
Related Product 1: CDC25C-3 blocking peptide
Related Product 2: CDC25C-2 pan-specific antibody (Cat. No.: AB-NP003-2)
Antibody Cross Reactivity: Weak immunoreactivity on protein dot blots with recombinant human CDC25A, and very weak immunoreactivity with recombinant human CDC25B.
Related Product 1: CDC25C-3 blocking peptide
Related Product 2: CDC25C-2 pan-specific antibody (Cat. No.: AB-NP003-2)
Scientific Background: CDC25C is a protein-tyrosine phosphatase that functions as a dosage-dependent inducer of mitotic progression. When phosphorylated, it is highly effective in activating G2 cells into prophase. It directly dephosphorylates CDK1 and activates its kinase activity.
Figure 1. Dot blotting CDC25C-3 antibody with recombinant purified proteins.
© Kinexus Bioinformatics Corporation 2017