Product Name: CDC25B-3
Product Number: AB-NP002-2
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Cell division cycle 25B phosphatase
Target Alias: CDC25HU2; CDC25M2; Cell division cycle 25 B; M-phase inducer phosphatase 2; MPIP2
Product Type Specific: Protein phosphatase pan-specific antibody
Antibody Code: NP002-2
Antibody Target Type: Pan-specific
Protein UniProt: P30305
Protein SigNET: P30305
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDC25B sequence peptide Cat. No.: PE-01ASY75
Antibody Immunogen Sequence: CAFKDELKTFRLKTR
Target Alias: CDC25HU2; CDC25M2; Cell division cycle 25 B; M-phase inducer phosphatase 2; MPIP2
Product Type Specific: Protein phosphatase pan-specific antibody
Antibody Code: NP002-2
Antibody Target Type: Pan-specific
Protein UniProt: P30305
Protein SigNET: P30305
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDC25B sequence peptide Cat. No.: PE-01ASY75
Antibody Immunogen Sequence: CAFKDELKTFRLKTR
Antibody Immunogen Description: Corresponds to amino acid residues A549 to R562
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with recombinant human CDC25B on protein dot blots.
Antibody Species Reactivity: Human; Mouse
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with recombinant human CDC25B on protein dot blots.
Antibody Species Reactivity: Human; Mouse
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 62-68 kDa.
Antibody Specificity: Medium
Antibody Cross Reactivity: Strong immunoreactivity on protein dot blots with recombinant human CDC25C, but no immunoreactivity with recombinant human CDC25A.
Related Product 1: CDC25B-3 blocking peptide
Related Product 2: CDC25B pan-specific antibody (Cat. No.: AB-NP002-2P)
Related Product 3: CDC25B-1 pan-specific antibody (Cat. No.: AB-NP002-3)
Related Product 4: CDC25B-4 pan-specific antibody (Cat. No.: AB-NP002-3)
Antibody Specificity: Medium
Antibody Cross Reactivity: Strong immunoreactivity on protein dot blots with recombinant human CDC25C, but no immunoreactivity with recombinant human CDC25A.
Related Product 1: CDC25B-3 blocking peptide
Related Product 2: CDC25B pan-specific antibody (Cat. No.: AB-NP002-2P)
Related Product 3: CDC25B-1 pan-specific antibody (Cat. No.: AB-NP002-3)
Related Product 4: CDC25B-4 pan-specific antibody (Cat. No.: AB-NP002-3)
Scientific Background: CDC25B is a protein-tyrosine phosphatase that functions as a dosage-dependent inducer of mitotic progression. It is required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. It directly dephosphorylates CDK1 and stimulates its kinase activity.
Figure 1. Dot blotting CDC25B-3 antibody with recombinant purified proteins.
Figure 2. Immunoblotting of various cell lines with NP002-2 antibody at a 1000 X dilution. The target protein CDC25B is indicated. The expected molecular masses of CDC25B range from 61-67 kDa. Each lane was loaded with 20 µg of cell lysate protein.
© Kinexus Bioinformatics Corporation 2017