Target Full Name: Autophagy-related cysteine endopeptidase 1

Target Alias: MGC1353; ATG4B; hAPG4B; AUT-like 1 cysteine endopeptidase; Cysteine protease ATG4B; Autophagy-related protein 4 homologue B; Autophagin-1; EC:3.4.22.-; ATG4B_HUMAN; APG4B; APG4 autophagy 4 homologue B; AUTL1; KIAA0943; Autophagy related cysteine endopeptidase 1; Autophagin 1; ATG4 autophagy related 4 homologue B (S. cerevisiae); AUT like 1 cysteine endopeptidase; Autophagy related protein 4 homologue B;

Product Type Specific: Autophagy protein pan-specific antibody

Antibody Code: NN374-1

Antibody Target Type: Pan-specific

Protein UniProt: Q9Y4P1

Protein SigNET: Q9Y4P1

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Synthetic peptide from the N-terminal to mid of Human ATG4B (AA 94-106)

Production Method: Peptide Affinity purified

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting

Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.

Antibody Potency: Detects a medium ~44 kDa protein band in mouse brain lysates by Western blotting.

Antibody Species Reactivity: Human | Mouse

Antibody Positive Control: A 1:1000 dilution of SPC-630 was sufficient for detection of ATG4B in 15 µg of Human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Antibody Specificity: Very high

Antibody Cross Reactivity: No other prominent cross-reactive proteins in mouse brain lysates.