Product Name: ATG4A
Product Number: AB-NN373-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: ATG4 autophagy related 4 homologue A (S. cerevisiae)
Target Alias: MGC107179; AV169859; Autophagy-related cysteine endopeptidase 2; hAPG4A; EC:3.4.22.-; ATG4A; Autophagin 2; Autophagy related protein 4 homologue A; Cysteine protease ATG4A; AUTL2; Autl2; Autophagy-related protein 4 homologue A; Atg4al; Autophagin-2; Autophagy related 4A cysteine peptidase; AUT like 2 cysteine endopeptidase; Autophagy related cysteine endopeptidase 2; Apg4a; AUT-like 2 cysteine endopeptidase; APG4A; AI627006; ATG4A_HUMAN;
Product Type Specific: Autophagy protein pan-specific antibody
Antibody Code: NN373-1
Antibody Target Type: Pan-specific
Protein UniProt: Q8WYN0
Protein SigNET: Q8WYN0
Target Alias: MGC107179; AV169859; Autophagy-related cysteine endopeptidase 2; hAPG4A; EC:3.4.22.-; ATG4A; Autophagin 2; Autophagy related protein 4 homologue A; Cysteine protease ATG4A; AUTL2; Autl2; Autophagy-related protein 4 homologue A; Atg4al; Autophagin-2; Autophagy related 4A cysteine peptidase; AUT like 2 cysteine endopeptidase; Autophagy related cysteine endopeptidase 2; Apg4a; AUT-like 2 cysteine endopeptidase; APG4A; AI627006; ATG4A_HUMAN;
Product Type Specific: Autophagy protein pan-specific antibody
Antibody Code: NN373-1
Antibody Target Type: Pan-specific
Protein UniProt: Q8WYN0
Protein SigNET: Q8WYN0
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide from the mid-protein of Human ATG4A (AA 102-113)
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide from the mid-protein of Human ATG4A (AA 102-113)
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a medium ~45 kDa protein band in mouse brain lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: A 1:1000 dilution of SPC-628 was sufficient for detection of ATG4A in 15 µg of Human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: No other prominent cross-reactive proteins in mouse brain lysates.
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a medium ~45 kDa protein band in mouse brain lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: A 1:1000 dilution of SPC-628 was sufficient for detection of ATG4A in 15 µg of Human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: No other prominent cross-reactive proteins in mouse brain lysates.
Scientific Background: Autophagy related 4A, cysteine peptidase (ATG4A or APG4A) is a C-54 cysteine protease that also belongs to the family of autophagin proteins, and is primarily found in skeletal muscle and brain. ATG4A is involved in cellular autophagy and has been studied as a target for cancer therapy. Specifically it cleaves GATE-16. Mutations in the ATG4a gene is critical for redox regulation and inhibits autophagosome formation.
References
[1] Mariño G. et al. (2003) J Biol Chem. 278: 3671-8.
[2] Scherz-Shouval R. et al. (2003) J Biol Chem. 278: 14053-8.
[3] Li M. et al. (2011) J Biol Chem. 286: 7327-38.
[4] Scherz-Shouval R. et al. (2007) EMBO J. 26: 1749-60.
[1] Mariño G. et al. (2003) J Biol Chem. 278: 3671-8.
[2] Scherz-Shouval R. et al. (2003) J Biol Chem. 278: 14053-8.
[3] Li M. et al. (2011) J Biol Chem. 286: 7327-38.
[4] Scherz-Shouval R. et al. (2007) EMBO J. 26: 1749-60.
© Kinexus Bioinformatics Corporation 2017