Product Name: Tsp23
Product Number: AB-NN354-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: PTGES3L
Target Alias: Transcript similar p23; AARSD1; AASD1_HUMAN; Alanyl-tRNA editing protein Aarsd1; Alanyl-tRNA synthetase domain containing 1; Alanyl-tRNA synthetase domain-containing protein 1; MGC2744; OTTHUMP00000204017; OTTHUMP00000204019; OTTHUMP00000204020
Product Type Specific: Tsp23 pan-specific antibody
Antibody Code: NN354-1
Antibody Target Type: Pan-specific
Protein UniProt: B9A003
Protein SigNET: B9A003
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1 Kappa
Target Alias: Transcript similar p23; AARSD1; AASD1_HUMAN; Alanyl-tRNA editing protein Aarsd1; Alanyl-tRNA synthetase domain containing 1; Alanyl-tRNA synthetase domain-containing protein 1; MGC2744; OTTHUMP00000204017; OTTHUMP00000204019; OTTHUMP00000204020
Product Type Specific: Tsp23 pan-specific antibody
Antibody Code: NN354-1
Antibody Target Type: Pan-specific
Protein UniProt: B9A003
Protein SigNET: B9A003
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1 Kappa
Antibody Immunogen Source: Recombinant tsp23
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~19 kDa protein in cell and tissue lysates by Western blotting. Detects a Tsp23.
Antibody Species Reactivity: Human
Antibody Positive Control: 1 µg/ml of SMC-194 was sufficient for detection of tsp23 in 20 µg of transiently transfected Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Cross Reactivity: No cross-reactivity to p23.
Antibody Species Reactivity: Human
Antibody Positive Control: 1 µg/ml of SMC-194 was sufficient for detection of tsp23 in 20 µg of transiently transfected Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Cross Reactivity: No cross-reactivity to p23.
Scientific Background: p23 is a highly conserved ubiquitous protein, known to have an important function as a cochaperone for the HSP90 chaperoning system (1). Studies have revealed that p23 is a small protein (18 to 25 kDa) with a simple structure (2, 3). p23 is a phosphor-protein, which is highly acidic and has an aspartic acid-rich c-terminal domain (1). Numerous studies have found p23 to be associated with other client proteins like Fes tyrosine kinase (4), the heme regulated kinase HRI (5), hsf1 transcription factor (4), aryl hydrocarbon receptor (4), telomerase (6), and Hepadnavirus reverse transcriptase (7). In spite of several years of study, the exact functional significance of p23 is still not clear (8). p23 is thought to be involved in the adenosine triphosphate–mediated HSP90 binding of client proteins (8). Since many HSP90 client proteins are involved in oncogenic survival signaling, a recent study has concluded p23 to be a promising target in leukemic apoptosis (9). HSP90 and its co-chaperone p23 are certainly among the emerging anti-tumor targets in oncology. Specifically TSP23 (transcript similar p23) displays 44% and 17% amino acid identity with p23 and Sba1p respectively (10).
References
[1] Johnson J.L., Beito T. G., Krco C.J., Toft D.O. (1994) Mol Cell Biol 14: 1956-63.
[2] Weikl T., Abelmann K., Buchner J. (1999) J Mol Biol 293: 685-91.
[3] Weaver A.J., Sullivan W.P., Felts S.J., Owen B.A., Toft D.O. (2000) J Biol Chem 275: 23045-52.
[4] Nair S.C. et al. (1996) Cell Stress Chaperones 1: 237-50.
[5] Xu Z. et al. (1997) Eur J Biochem 246, 461-70.
[6] Holt S.E. et al. (1999) Genes Dev 13: 817-26.
[7] Hu J., Toft D., Anselmo D., Wang X. (2002) J Virol 76: 269-79.
[8] Felts, S.J., Toft D.O. (2003) Cell Stress Chaperones.8: 108-13.
[1] Johnson J.L., Beito T. G., Krco C.J., Toft D.O. (1994) Mol Cell Biol 14: 1956-63.
[2] Weikl T., Abelmann K., Buchner J. (1999) J Mol Biol 293: 685-91.
[3] Weaver A.J., Sullivan W.P., Felts S.J., Owen B.A., Toft D.O. (2000) J Biol Chem 275: 23045-52.
[4] Nair S.C. et al. (1996) Cell Stress Chaperones 1: 237-50.
[5] Xu Z. et al. (1997) Eur J Biochem 246, 461-70.
[6] Holt S.E. et al. (1999) Genes Dev 13: 817-26.
[7] Hu J., Toft D., Anselmo D., Wang X. (2002) J Virol 76: 269-79.
[8] Felts, S.J., Toft D.O. (2003) Cell Stress Chaperones.8: 108-13.
[9] Gausdal G., Gjertsen B.T., Fladmark K.E., Demol H., Vandekerckhove J. & Doskeland S.O. (2004) Leukemia
[10] Freeman B.C., Felts S.J., Toft D.O., Yamamato K.R. (2000) Genes Dev. 14(4): 433-434.
[10] Freeman B.C., Felts S.J., Toft D.O., Yamamato K.R. (2000) Genes Dev. 14(4): 433-434.
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