Product Name: TRAP1
Product Number: AB-NN349-2
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Heat shock protein 75 kDa, mitochondrial
Target Alias: Heat shock protein 75; Heat shock protein 75 kDa; HSP 75; HSP 90L; HSP75; HSP90L; mitochondrial; TNF receptor associated protein 1; TNFR associated protein 1; TNFR-associated protein 1; TRAP 1; TRAP-1; Trap1; TRAP1_HUMAN; Tumor necrosis factor receptor associated protein; Tumor necrosis factor type 1 receptor associated protein; Tumor necrosis factor type 1 receptor-associated protein
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN349-2
Antibody Target Type: Pan-specific
Protein UniProt: Q12931
Protein SigNET: Q12931
Antibody Type: Polyclonal
Target Alias: Heat shock protein 75; Heat shock protein 75 kDa; HSP 75; HSP 90L; HSP75; HSP90L; mitochondrial; TNF receptor associated protein 1; TNFR associated protein 1; TNFR-associated protein 1; TRAP 1; TRAP-1; Trap1; TRAP1_HUMAN; Tumor necrosis factor receptor associated protein; Tumor necrosis factor type 1 receptor associated protein; Tumor necrosis factor type 1 receptor-associated protein
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN349-2
Antibody Target Type: Pan-specific
Protein UniProt: Q12931
Protein SigNET: Q12931
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Human recombinant TRAP1 (amino acids 1-59 removed - transit peptide)
Production Method: Protein A purified.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.09% azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Human recombinant TRAP1 (amino acids 1-59 removed - transit peptide)
Production Method: Protein A purified.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.09% azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:120); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~75 kDa protein, multiple isoforms.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SPC-209 was sufficient for detection of Trap-1 in 20 µg of Hela cell lysate by ECL immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: High
Antibody Cross Reactivity: Two other prominent ~65 and 20 kDa cross-reactive proteins in mouse brain lysates.
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:120); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~75 kDa protein, multiple isoforms.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SPC-209 was sufficient for detection of Trap-1 in 20 µg of Hela cell lysate by ECL immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: High
Antibody Cross Reactivity: Two other prominent ~65 and 20 kDa cross-reactive proteins in mouse brain lysates.
Related Product 1: TRAP1 pan-specific antibody (Cat. No.: AB-NN349-1)
Related Product 2: TRAP1 pan-specific antibody (Cat. No.: AB-NN349-2)
Related Product 2: TRAP1 pan-specific antibody (Cat. No.: AB-NN349-2)
Scientific Background: The 90 kDa heat shock protein (HSP90) family of molecular chaperones is a highly conserved family of proteins that play an important physiological role. HSP90 is involved in numerous cellular processes but is best known for its association with signal transduction machinery. A recently cloned homolog of HSP90 is the tumor necrosis factor receptor-associated protein (TRAP1). Like HSP90, TRAP1 is found to beassociated with numerous proteins involved in diverse actions (1, 2). Immunofluorescence data has shown TRAP1 to be localized in the mitochondria of mammalian cells. This observation and the fact that TRAP1 is shown to have a mitochondrial targeting presequence strongly implicates TRAP1 as a mitochondrial matrix protein (3).
References
[1] Felts S.J. et al. (2000) J Biol Chem. 275(5): 3305-3312.
[2] Costantino E. et al. (2009) Cancer Lett. 279(1): 39-46.
[3] Cechetto J.D., Gupta R.S. (2000) Exp Cell Res. 260(1): 30-39.
[1] Felts S.J. et al. (2000) J Biol Chem. 275(5): 3305-3312.
[2] Costantino E. et al. (2009) Cancer Lett. 279(1): 39-46.
[3] Cechetto J.D., Gupta R.S. (2000) Exp Cell Res. 260(1): 30-39.
© Kinexus Bioinformatics Corporation 2017