Product Name: TRAP1
Product Number: AB-NN349-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Heat shock protein 75 kDa, mitochondrial
Target Alias: Heat shock protein 75; Heat shock protein 75 kDa; HSP 75; HSP 90L; HSP75; HSP90L; mitochondrial; TNF receptor associated protein 1; TNFR associated protein 1; TNFR-associated protein 1; TRAP 1; TRAP-1; Trap1; TRAP1_HUMAN; Tumor necrosis factor receptor associated protein; Tumor necrosis factor type 1 receptor associated protein; Tumor necrosis factor type 1 receptor-associated protein
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN349-1
Antibody Target Type: Pan-specific
Protein UniProt: Q12931
Protein SigNET: Q12931
Antibody Type: Monoclonal
Target Alias: Heat shock protein 75; Heat shock protein 75 kDa; HSP 75; HSP 90L; HSP75; HSP90L; mitochondrial; TNF receptor associated protein 1; TNFR associated protein 1; TNFR-associated protein 1; TRAP 1; TRAP-1; Trap1; TRAP1_HUMAN; Tumor necrosis factor receptor associated protein; Tumor necrosis factor type 1 receptor associated protein; Tumor necrosis factor type 1 receptor-associated protein
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN349-1
Antibody Target Type: Pan-specific
Protein UniProt: Q12931
Protein SigNET: Q12931
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1 Kappa
Antibody Immunogen Source: Purified recombinant TRAP1
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Ig Isotype Clone: IgG1 Kappa
Antibody Immunogen Source: Purified recombinant TRAP1
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~75 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Rat
Antibody Positive Control: 1 µg/ml of SMC-207 was sufficient for detection of Trap-1/HSP75 in 20 µg of Human A431 lysate by ECL immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Related Product 1: TRAP1 pan-specific antibody (Cat. No.: AB-NN349-3)
Related Product 2: TRAP1 pan-specific antibody (Cat. No.: AB-NN349-2)
Antibody Potency: Detects a ~75 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Rat
Antibody Positive Control: 1 µg/ml of SMC-207 was sufficient for detection of Trap-1/HSP75 in 20 µg of Human A431 lysate by ECL immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Related Product 1: TRAP1 pan-specific antibody (Cat. No.: AB-NN349-3)
Related Product 2: TRAP1 pan-specific antibody (Cat. No.: AB-NN349-2)
Scientific Background: The 90 kDa heat shock protein (HSP90) family of proteins that play an important physiological role. HSP90 is involved in numerous cellular processes but is best known for its association with signal transduction machinery. A recently cloned homolog of HSP90 is the tumor necrosis factor receptor-associated protein (TRAP1). Like HSP90, TRAP1 is found to be associated with numerous proteins involved in diverse actions (1, 2). Immunofluorescence data has shown TRAP1 to be localized in the mitochondria of mammalian cells. This observation and the fact that TRAP1 is shown to have a mitochondrial targeting pre-sequence strongly implicates TRAP1 as a mitochondrial matrix protein (3).
References
[1] Felts S.J. et al. (2000) J Biol Chem. 275(5): 3305-3312.
[2] Costantino E. et al. (2009) Cancer Lett. 279(1): 39-46.
[3] Cechetto J.D., Gupta R.S. (2000) Exp Cell Res. 260(1): 30-39.
[1] Felts S.J. et al. (2000) J Biol Chem. 275(5): 3305-3312.
[2] Costantino E. et al. (2009) Cancer Lett. 279(1): 39-46.
[3] Cechetto J.D., Gupta R.S. (2000) Exp Cell Res. 260(1): 30-39.
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