Product Name: Trak2
Product Number: AB-NN348-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Trafficking kinesin-binding protein 2
Target Alias: Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 3 protein; GABA-A receptor-interacting factor 1; O-GlcNAc transferase-interacting protein of 98 kDa; EMBL AAH60681.1; Ensembl ENSMUSP00000027186 MGI 1918077; Als2cr3; Grif1; Oip98; KIAA0549; TRAK2_Human
Product Type Specific: Trak2 pan-specific antibody
Antibody Code: NN348-1
Antibody Target Type: Pan-specific
Protein UniProt: O60296
Protein SigNET: O60296
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Target Alias: Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 3 protein; GABA-A receptor-interacting factor 1; O-GlcNAc transferase-interacting protein of 98 kDa; EMBL AAH60681.1; Ensembl ENSMUSP00000027186 MGI 1918077; Als2cr3; Grif1; Oip98; KIAA0549; TRAK2_Human
Product Type Specific: Trak2 pan-specific antibody
Antibody Code: NN348-1
Antibody Target Type: Pan-specific
Protein UniProt: O60296
Protein SigNET: O60296
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2A
Antibody Immunogen Source: Fusion protein amino acids 757-914 (C-terminus) of human TRAK2
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Immunogen Source: Fusion protein amino acids 757-914 (C-terminus) of human TRAK2
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~100 kDa protein by Western blotting.
Antibody Species Reactivity: Human | Rat
Antibody Positive Control: A 1:100 dilution of SMC-483 was sufficient for detection of Trak2 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: Does not cross-react with TRAK1.
Antibody Potency: In mouse brain lysates, this antibody detects a ~100 kDa protein by Western blotting.
Antibody Species Reactivity: Human | Rat
Antibody Positive Control: A 1:100 dilution of SMC-483 was sufficient for detection of Trak2 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: Does not cross-react with TRAK1.
Scientific Background: TRAK2, a member of the TRAK family of adaptor proteins, links mitochondria to microtubule based motors. Localized in dendrites, it is specifically required for dendritic development as it reacts with dynein and dynactin. TRAK2 interferes with kinesin-1 binding and axonal transport (1). It has been shown to interact with Kir2.1 and GABA-R Beta2 (2, 3).
References
[1] van Spronsen M. et al. (2013) Neuron. 77(3): 48-502.
[2] Grishin A., Li H., Levitan E.S., Zaks E. (2006) J Biol Chem. 281(4): 30104-30111.
[3] Beck M. et al. (2002) J Biol Chem. 277(33): 30079-30090.
[1] van Spronsen M. et al. (2013) Neuron. 77(3): 48-502.
[2] Grishin A., Li H., Levitan E.S., Zaks E. (2006) J Biol Chem. 281(4): 30104-30111.
[3] Beck M. et al. (2002) J Biol Chem. 277(33): 30079-30090.
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