Product Name: TCP1-alpha
Product Number: AB-NN346-2
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: T-complex protein 1 subunit alpha
Target Alias: p63; Tcp-1; TCP1 alpha; Ccta; TRic; Tp63; 21454; c-cpn; CCT; Tcp1; ccpn; AI528772; Cct1; Tcp 1; c cpn
Product Type Specific: Chaperone protein pan-specific antibody
Antibody Code: NN346-2
Antibody Target Type: Pan-specific
Protein UniProt: P17987
Protein SigNET: P17987
Antibody Type: Monoclonal
Antibody Host Species: Rat
Antibody Ig Isotype Clone: IgG2c
Target Alias: p63; Tcp-1; TCP1 alpha; Ccta; TRic; Tp63; 21454; c-cpn; CCT; Tcp1; ccpn; AI528772; Cct1; Tcp 1; c cpn
Product Type Specific: Chaperone protein pan-specific antibody
Antibody Code: NN346-2
Antibody Target Type: Pan-specific
Protein UniProt: P17987
Protein SigNET: P17987
Antibody Type: Monoclonal
Antibody Host Species: Rat
Antibody Ig Isotype Clone: IgG2c
Antibody Immunogen Source: Purified recombinant mouse TCP1 alpha construct encoding the C-terminal half of the 1.8 kb full-length Tcp 1b gene expressed in E. coli. Detects the COOH group.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence | Immunoprecipitation
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~60 kDa protein in cell and tissue lysates by Western blotting. Also detects ~92 kDa protein in cell and tissue lysates by Western blotting. The addition of an alanine (LDDA COOH) prevents binding in ELISA assays to immobilized synthetic peptide sequences. This antibody recognizes other proteins, most notably the p102B' COP subunit of Golgi coatomer. It does not react with human HSP60 protein.
Antibody Species Reactivity: Mouse | Rat | Sheep | Rabbit | Hamster | Dog | Bovine
Antibody Positive Control: 1 µg/ml of SMC-478 was sufficient for detection of TCP1 alpha in 20 µg of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
Antibody Potency: Detects a ~60 kDa protein in cell and tissue lysates by Western blotting. Also detects ~92 kDa protein in cell and tissue lysates by Western blotting. The addition of an alanine (LDDA COOH) prevents binding in ELISA assays to immobilized synthetic peptide sequences. This antibody recognizes other proteins, most notably the p102B' COP subunit of Golgi coatomer. It does not react with human HSP60 protein.
Antibody Species Reactivity: Mouse | Rat | Sheep | Rabbit | Hamster | Dog | Bovine
Antibody Positive Control: 1 µg/ml of SMC-478 was sufficient for detection of TCP1 alpha in 20 µg of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
Scientific Background: T-complex polypeptide-1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is up-regulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL.
© Kinexus Bioinformatics Corporation 2017