Product Name: SOD (EC)
Product Number: AB-NN328-2
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Extracellular superoxide dismutase [Cu-Zn]
Target Alias: EC SOD; EC-SOD; Extracellular superoxide dismutase; MGC20077; SOD 3; SOD3; SODE_HUMAN; Superoxide dismutase 3 extracellular antibody
Product Type Specific: Superoxide dismutase pan-specific antibody
Antibody Code: NN328-2
Antibody Target Type: Pan-specific
Protein UniProt: P08294
Protein SigNET: P08294
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Peptide corresponding to AA 227-236 of human EC SOD
Target Alias: EC SOD; EC-SOD; Extracellular superoxide dismutase; MGC20077; SOD 3; SOD3; SODE_HUMAN; Superoxide dismutase 3 extracellular antibody
Product Type Specific: Superoxide dismutase pan-specific antibody
Antibody Code: NN328-2
Antibody Target Type: Pan-specific
Protein UniProt: P08294
Protein SigNET: P08294
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Peptide corresponding to AA 227-236 of human EC SOD
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4; 50% glycerol, 0.09% azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4; 50% glycerol, 0.09% azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a extracellular SOD ~35 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SPC-124 was sufficient for detection of ECSOD in 20 µg of Hela lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP asthe secondary antibody.
Related Product 1: SOD (EC) pan-specific antibody (Cat. No.: AB-NN328-1)
Related Product 2: SOD (Cu/Zn) pan-specific antibody (Cat. No.: AB-NN098-2)
Related Product 3: SOD (EC) pan-specific antibody (Cat. No.: AB-NN328-1)
Related Product 4: SOD (Mn) pan-specific antibody (Cat. No.: AB-NN068-1)
Related Product 5: SOD (Mn) pan-specific antibody (Cat. No.: AB-NN068-2)
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SPC-124 was sufficient for detection of ECSOD in 20 µg of Hela lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP asthe secondary antibody.
Related Product 1: SOD (EC) pan-specific antibody (Cat. No.: AB-NN328-1)
Related Product 2: SOD (Cu/Zn) pan-specific antibody (Cat. No.: AB-NN098-2)
Related Product 3: SOD (EC) pan-specific antibody (Cat. No.: AB-NN328-1)
Related Product 4: SOD (Mn) pan-specific antibody (Cat. No.: AB-NN068-1)
Related Product 5: SOD (Mn) pan-specific antibody (Cat. No.: AB-NN068-2)
Related Product 6: SOD1 (UβB) pan-specific antibody (Cat. No.: AB-NN098-5)
Related Product 7: SOD1 (EDI) pan-specific antibody (Cat. No.: AB-NN098-4)
Related Product 7: SOD1 (EDI) pan-specific antibody (Cat. No.: AB-NN098-4)
Scientific Background: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).
References
[1] Adachi T. et al. (1992). Clin. Chim. Acta. 212: 89-102.
[2] Barrister J.V. et al. (1987). Crit. Rev. Biochem. 22:111-180.
[3] Furukawa Y., O’Halloran T. (2006). Antioxidants & Redo Signaling. Vol 8, No 5,6.
[4] Gao B. et al. (2003). Am J Physiol Lung Cell Mol Physiol 284: L917-L925.
[5] Hassan H.M. (1988). Free Radical Biol. Med. 5: 377-385.
[6] Kurobe N. et al. (1990) Biomedical Research. 11: 187-194
[7] Wispe J.R. et al. (1989) BBA. 994: 30-36.
[8] Regan, E. et al. (2005) Arthritis & Rheumatism 52(11): 3479–3491
[1] Adachi T. et al. (1992). Clin. Chim. Acta. 212: 89-102.
[2] Barrister J.V. et al. (1987). Crit. Rev. Biochem. 22:111-180.
[3] Furukawa Y., O’Halloran T. (2006). Antioxidants & Redo Signaling. Vol 8, No 5,6.
[4] Gao B. et al. (2003). Am J Physiol Lung Cell Mol Physiol 284: L917-L925.
[5] Hassan H.M. (1988). Free Radical Biol. Med. 5: 377-385.
[6] Kurobe N. et al. (1990) Biomedical Research. 11: 187-194
[7] Wispe J.R. et al. (1989) BBA. 994: 30-36.
[8] Regan, E. et al. (2005) Arthritis & Rheumatism 52(11): 3479–3491
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